FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004047448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1132109
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT1034689.139402654691377No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA421643.724376363053381No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA141631.251027948722252No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC127281.124273369437042No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG123531.0911493504600707No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC111190.9821492453465169No Hit
GTTTAGCTCTCATCTTCAAGGTCCTTCATTTCTACATCCTGGGGGGCTTT110910.9796759852629032No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82750.7309366854251667No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79450.7017875487254319No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT74820.6608904266285314No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG72600.6412810073941644No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA70850.6258231318715777No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT70840.6257348011543058No Hit
CCTGATGTCCTCGCCAACATTGGTGCCTCCAATGCAGGCATGGCACTGGA69730.6159300915371223No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC63830.5638149683466874No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG59200.5229178462497869No Hit
GCTACAGCCATGTTTTCTCTGCTCTTTCCTCAATGTGGGATCCTTCATGT54090.47778084972383406No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC45990.406232968733576No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA42920.3791154385310955No Hit
ATATGGCCCCATGTGATCTGGTTCCTGCTACCTCTTCAATCTCTTGGCCT42830.3783204620756482No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC40310.35606112132312345No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC39930.3527045540667904No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC36840.32541036242976606No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC34120.3013844073318029No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA32900.29060805982462823No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA32240.2847782324846812No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31930.28203998024925164Clontech SMART CDS Primer II A (100% over 21bp)
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG28090.248120984816833No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT27980.24714934692684185No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT26480.23389973933605332No Hit
GAATTCCCGTAGAAGCTTAAGGTTGTAAGCTGGGAGCAGTGGCTCAGGGA26360.23283977072879025No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG25740.227363266257931No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG25020.2210034546143525No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA23880.21093375284535323No Hit
GTGTGCTTGTGATGATACACACACATGCACAGGTTCTCAACCACGTGTGT23800.21022710710717785No Hit
GTTCCATGGGAGAGTATTTTAGAGACAATGGCAAACATGCTTTGATCATC23790.2101387763899059No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG23740.2096971228035463No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC23370.20642888626448513No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT21880.19326760939096854No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC21520.19008770356917928No Hit
GCCGAGAACTTGCTTCAAATACTGGAAATCAGCGTAGTCGCCAGAGGCAC19660.17365819015660153No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG19560.1727748829838823No Hit
GTATCAACGCAGAGTACATGGGGATCCGGAGGGATCACTTCAATTTGTGT18880.1667683942093915No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG18250.16120355902126032No Hit
AACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATT16720.14768895927865605No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16550.14618733708503334No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA16460.14539236062958602No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG15890.14035750974508637No Hit
CGTCAGATGTGTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCAC15770.1392975411378233No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC15140.1337327059496921No Hit
TGCTTCCGGATAACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA14620.1291395086515521No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA14450.12763788645792942No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG14310.1264012564161225No Hit
CTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATC13580.11995311405527206No Hit
GAATTTACTCCCGTGCCATAAGTTTTTGTTTCTTCAGTTTCTTCTGGGAT13540.11959979118618437No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACCTGTCTCT12740.11253333380443048No Hit
GGTCTACCTCTCATCCAGGTCCTGAAACTCCATTCTTGTGCCTTAGAGGA12330.10891177439628164No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG11980.1058201992917643No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT11940.10546687642267662No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG11510.1016686555799839No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)