FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004047449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences590041
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA476238.07113403983791No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA269874.57374995974856No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA93951.5922622326245126No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG86231.4614238671549942No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC77461.3127901281436376No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC67021.1358532712133562No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC53210.9018017392011742No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51750.8770576959906176No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA50880.8623129579130941No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT49650.8414669489069404No Hit
CCTGAAGAGAGCAGGACAAGCAGCTGTGCATGCTCAAATAGCAAGACGTG49220.8341793197421874No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT48160.8162144664523313No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG46780.7928262612259148No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45190.7658789812911306No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC44100.7474056887572219No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC33600.5694519533388358No Hit
CAGCCTGGCGGTGCACCCTGCAGGTGACAACGTCATCTGTGGGAGCTACG32700.5541987760172599No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC31410.532335888523001No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA28280.4792887273935201No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC24750.4194623763433388No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC23450.39743000910106246No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG22210.37641452034689116No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG18520.3138764933284297No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT18380.3115037768561846No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA17990.30489406668350166No Hit
AACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATT16870.28591233490554047No Hit
GATGAGCACCAGACTTAAGCTGGAGACACACTGATTCTCCTGTTTCTGGG16680.2826922196932078No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA15920.2698117588438769No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC15650.2652358056474042No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14610.24760991185358308No Hit
GTATCAACGCAGAGTACATGGGGATCCGGAGGGATCACTTCAATTTGTGT14520.24608459412142547No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG14040.23794956621658497No Hit
CTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATC13760.23320413327209466No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG13590.23032297755579698No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG11980.20303673812497774No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11560.1959185887082423Clontech SMART CDS Primer II A (100% over 21bp)
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACA11490.19473223047211974No Hit
GTACATGGGGATCCGGAGGGATCACTTCAATTTGTGTTCTGGTGCTATCC11190.1896478380315944No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACCTGTCTCT10800.1830381278589115No Hit
CTGGTATTCTTAGCTTGCTTCACCTGGCATAGAATCTCTACCCTACAAGT10780.18269916836287647No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG10470.17744529617433363No Hit
CGTCAGATGTGTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCAC10360.17558101894614103No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC10230.17337778222191338No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA10060.17049662650561573No Hit
GGTATCAACGCAGAGTACATGGGGATCCGGAGGGATCACTTCAATTTGTG9720.16473431507302033No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG9600.16270055809681022No Hit
GGTATCAACGCAGAGTACATGGGGGCGGAAGGCGGACGACTAGAGTCGTT9520.16134472011267012No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG9330.15812460490033745No Hit
ACACTACCTTGAACTCGGCATCAGAGGGAGACCTGTCTCTTATACACATC9090.1540570909479172No Hit
ATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATTGAAGT9080.15388761119989966No Hit
CGTGGTTTTTCTGCCTCAGCCTCCTGAGTAGCTAAGGGAGGTGTCTTGAG8730.14795582001928678No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT8620.14609154279109418No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG8530.14456622505893657No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC8500.14405778581488407No Hit
CCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGA7700.1304994059734832No Hit
TGCTTCCGGATAACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA7080.11999166159639754No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAA7050.119483222352345No Hit
AGATCAACCTCTGCTGGTCAGGAGGGATGCCTTCCTTGTCTTGGATCTTT6710.11372091091974965No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT6540.11083975520345196No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG6390.10829755898318931No Hit
GGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACA6380.10812807923517179No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCTG6360.10778911973913677No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTCTG6340.10745016024310175No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT6260.10609432225896166No Hit
CGTGGTTTTTCTGCCACAGCCTCTGGAGCCTGTCTCTTATACACATCTCC6100.10338264629068149No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)