FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004047454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1097815
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC859847.832285038918214No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA292142.6611041022394484No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC137321.2508482758934794No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA98210.8945951731393722No Hit
CCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGAT88050.8020477038480982No Hit
CAATAATGCAGACACCTCTGAACCAGCCTGGGTGAAGCGAAAGATGTTAT87190.7942139613687187No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG83900.7642453418836507No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT77230.7034882926540446No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75970.6920109490214653No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA74970.6829019461384659No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT73320.6678720913815169No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70450.6417292531073087No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC69980.6374480217522989No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC68090.62023200630343No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG56210.512017052053397No Hit
GCAGCGTCAGATGTGTATAAGAGACAGGACCTTGGTGAATGGCTCACCAG51540.4694780085897897No Hit
CTGCTGTCTTCCGTGGTCGGATGTCCATGAAGGAGGTCGATGAGCAGATG47750.4349548876632219No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC44240.402982287543894No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC42060.3831246612589553No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG39590.36062542413794674No Hit
GGGCTGGGGAACCTCCAGGACGGGGGAGGGGCCGCAGCCTGTCCTGGGGG37650.3429539585449279No Hit
TACTTCCAAGTTGTAGTGTTGTTGTTTTCAGCCTGCTGCTGCTGCTGCTG37150.33839945710342817No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC34660.3157180399247596No Hit
GAGTACATGGGCTGGGACCCGATCCGTCACCGCACCGTCGCGGACTTCTC33250.30287434585973044No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC31980.2913059121983212No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT30360.27654932752786215No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC30020.2734522665476424No Hit
ATCAACGCAGAGTACATGGGGAGATAGGCGGGAGCGAGGGAGCGAGGGAG29210.26607397421241286No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG28670.26115511265559316No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA28050.25550753086813355No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27260.24831141859056396Clontech SMART CDS Primer II A (100% over 21bp)
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC26650.24275492683193434No Hit
ACTTCAGGGTATCCGGGAGTCTTAGCTACAAATTGTCCAATACCCTCAGG25640.23355483392010495No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG25460.23191521340116505No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA25040.22808943219030528No Hit
GCGGTCCGCCGCGAGGCGAGCCACCGCCCGTCCCCGCCCCTTGCCTCTCG24790.22581218146955542No Hit
TCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGAT23060.21005360648196644No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG22660.2064100053287667No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG21160.19274650100426757No Hit
CCAAATACACTCTTGGGATCCCTGATTCCAGGACTTGATTGTTAGATGGC20860.19001380013936775No Hit
TCTCGATTCCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGA20430.186096928899678No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA20260.1845483984095681No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA19740.1798117169104084No Hit
AGGCAACCCTCGATCATAGAATCCGGCTTGGTGAGATCGGAAGAGCACAC18800.17124925420038897No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA18000.1639620518939894No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC17390.15840556013535978No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA17070.15549067921279996No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG16270.14820347690640046No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16230.14783911679108047No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA15700.14301134526309078No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT15520.14137172474415088No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG14940.13608850307201123No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC14660.1335379822647714No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC14370.13089637142870156No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA14290.1301676511980616No Hit
TGCTTCCAAGTTGTAGTGTTGTTGTTTTCAGCCTGCTGCTGCTGCTGCTG14200.12934784093859164No Hit
AACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATT13850.12615968992954185No Hit
GTATCAACGCAGAGTACATGGGGATCCGGAGGGATCACTTCAATTTGTGT13790.12561314975656188No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG13780.1255220597277319No Hit
AGAGACTTCCTCCACTGCCTCTGCCTGTCTCTTATACACATCTCCGAGCC13780.1255220597277319No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC13540.12333589903581205No Hit
TGCTTCCGGATAACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA13010.11850812750782236No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC12780.1164130568447325No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA12380.11276945569153272No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG12370.11267836566270274No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT12290.11194964543206277No Hit
GGATGCCTGTAGTCCCGGCTACTTGGGAGGCTGAGGCAGGAGAATGGCGT11780.10730405396173308No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG11300.10293173257789337No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)