FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004047455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences914779
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT827129.041746695103408No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA468275.118941296203783No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA167111.8267800200922846No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC104001.1368866141439626No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG95591.0449518408271288No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG81300.8887392474029245No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66130.7229068441667332No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC64090.700606375966217No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63780.6972175793279033No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC48990.5355391848741609No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA42840.46830983221084No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA40370.4413087751249209No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT39370.43037717306584433No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC38120.4167126704919986No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA37440.4092791810918266No Hit
GAGTCATAACCCTCAAATGCCTCATAGAAGTATGAGTACGCAGTTTTCCA36170.3953960464767993No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT35000.3826060720676797No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA32650.35691680722884983No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC32390.3540745906934899No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG31660.346094521190364No Hit
CAGTAGTGCAATCTCAGCTCACTGCAACCTCTATCTTCCAGGTCCAAGCC29380.32117046849566944No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC27960.30564759357178073No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26960.29471599151270417Clontech SMART CDS Primer II A (100% over 21bp)
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC25930.2834564413918553No Hit
AACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATT24980.2730714194357326No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC22900.2503336871528533No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA22700.24814736674103802No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG22590.24694489051453958No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG21770.23798097682609679No Hit
GTACATGGGCAGAAATCACATTGCGTCACATCACTTTCTGACCATCGCAA21050.23011022334356165No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC20280.22169288975807272No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT20150.22027178149039275No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT19800.21644572076971597No Hit
GTACATGGGCAGAAATCACATTGCGTCAACATCACTTTCTGACCATCGCA19620.21447803239908217No Hit
GTATCAACGCAGAGTACATGGGGATCCGGAGGGATCACTTCAATTTGTGT19500.213166240151993No Hit
CTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATC19490.21305692413140223No Hit
CGTCAGATGTGTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCAC18480.2020160060517349No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC18030.19709678512515044No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG16900.18474407479839391No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT16870.18441612673662164No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG16420.17949690581003716No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACCTGTCTCT16350.1787316936659018No Hit
CAATTATATTACTACCACTGACATGACTTTCCAAAAAACACATAATTTGA16350.1787316936659018No Hit
ACACTACCTTGAACTCGGCATCAGAGGGAGACCTGTCTCTTATACACATC14180.1550101171977057No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG13610.14877910402403205No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG13060.14276672289153994No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13030.14243877482976763No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG12390.1354425495119586No Hit
GTACATGGGGATCCGGAGGGATCACTTCAATTTGTGTTCTGGTGCTATCC12130.1326003329765987No Hit
ATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATTGAAGT11900.1300860645030111No Hit
CCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGA11560.12636931980292507No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAGG11460.12527615959701743Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC11460.12527615959701743No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG11090.1212314668351591No Hit
TGCTTCCGGATAACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA10910.11926377846452532No Hit
GGTATCAACGCAGAGTACATGGGGATCCGGAGGGATCACTTCAATTTGTG10630.11620292988798388No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA10630.11620292988798388No Hit
GCAATCGCAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGC10120.11062781283785483No Hit
GCATGAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT9930.10855080844663029No Hit
GTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTC9850.10767628028190415No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCTGTCTCTTATACAC9830.10745764824072263No Hit
CGTGGTTTTTCTGCCACAGCCTCTGGAGCCTGTCTCTTATACACATCTCC9670.10570859191127036No Hit
AGAGACTTCCTCCACTGCCTCTGCCTGTCTCTTATACACATCTCCGAGCC9530.10417816762299965No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)