FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004047456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences175867
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA138167.855936588444677No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG64483.666407000744881No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA54653.107461888813706No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT52572.98919069524129No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC49042.7884708330727195No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA28231.6051902858410048No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC23551.33908010030307No Hit
CTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATC20521.1667908135124838No Hit
CGTCAGATGTGTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCAC19491.108223828233836No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACCTGTCTCT15800.8984061819443102No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC14950.8500742038017366No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCTG12330.7010979888210978No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTCTG11370.6465112840953675No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT11100.6311587733912559No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC10770.6123945936417862No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA10680.6072770900737489No Hit
AACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATT10380.5902187448469582No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC9870.561219557961414No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC9460.5379064861514667No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7390.42020390408661096No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA7100.4037141703673799No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6980.39689083227666366No Hit
CGTCAGATGTGTATAAGAGACAGCGTGGTTTTTCTGCCTCAGCCTCTGGA6930.39404777473886515No Hit
GTGTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCACCTGTCTCT6840.38893027117082796No Hit
CCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGA6820.3877930481557086No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT6810.3872244366481489No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATCTGTCTCT6780.3855186021254698No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTCTGTCTCT6670.3792638755423132No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA6550.37244053745159694No Hit
CGTCAGATGTGTATAAGAGACAGTGCTTCCGGATGACTGACGACTCTCAC6300.35822524976260467No Hit
GTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTA6070.3451471850887318No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC5630.3201282787561055No Hit
GATCACTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCG5560.3161479982031876No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGCTGTCTC5490.3121677176502698No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCTG5490.3121677176502698No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA5410.30761882558979226No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATCTGTCTC5360.30477576805199386No Hit
TCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCAC5270.2996582644839566No Hit
ACTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAAT5130.29169770337812095No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCT4810.2735021351362109No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACCTGTCTCTTA4780.2717963006135318No Hit
CGTGGTTTTTCTGCCACAGCCTCTGGAGCCTGTCTCTTATACACATCTCC4740.2695218545832931No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACCTGTCTCT4700.2672474085530543No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAA4670.2655415740303752No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACCTGTCT4660.26497296252281555No Hit
GTGTATAAGAGACAGGCTCCAGAGGCTGTCTCTTATACACATCTCCGAGC4420.251326286341383No Hit
ATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATTGAAGT4120.23426794111459226No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGCT4080.23199349508435352No Hit
GGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACA4020.22858182603899538No Hit
GATGTGTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCACCTGTC3970.22573876850119695No Hit
GTATAAGAGACAGCGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCT3770.2143665383500031No Hit
GGAGGGATCACTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGG3710.210954869304645No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCCTGTC3670.20868042327440622No Hit
GCGTCAGATGTGTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCA3570.20299430819880931No Hit
CCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACC3460.19673958161565272No Hit
TTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTG3350.19048485503249613No Hit
CAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGT3190.1813870709115411No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCTGTCTCT3180.18081845940398142No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTCT3150.17911262488130233No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCCT3090.17570095583594422No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCCTGTCTCT2960.16830900623766823No Hit
GTACATGGGGATCCGGAGGGATCACTTCAATTTGTGTTCTGGTGCTATCC2950.16774039473010854No Hit
GTATCAACGCAGAGTACATGGGGATCCGGAGGGATCACTTCAATTTGTGT2910.16546594869986977No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG2910.16546594869986977Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTT2890.1643287256847504No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCCTGTCTCT2860.16262289116207135No Hit
CGTGGTTTTTCTGCCTCCCGAACCAGAGCTGTCTCTTATACACATCTCCG2810.1597798336242729No Hit
GTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTC2760.15693677608647444No Hit
GGATCACTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTC2720.15466233005623567No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCTGTCTCTTATACAC2720.15466233005623567No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGC2710.15409371854867598No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACCTGTCTCT2580.14670176895040002No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC2510.1427214883974822No Hit
ACACTACCTTGAACTCGGCATCAGAGGGAGACCTGTCTCTTATACACATC2460.13987843085968374No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCCTGTCTCTTATAC2450.13930981935212405No Hit
GCAATCGCAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGC2440.13874120784456437No Hit
ATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTT2410.13703537332188528No Hit
CGTGGTTTTTCTGCCTCAGCCTCCCGAACCAGAGCTGTCTCTTATACACA2410.13703537332188528No Hit
CAGACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCAC2410.13703537332188528No Hit
GCAATCGCAGGCACTGACGACTCTCACCCTGTCTCTTATACACATCTCCG2400.1364667618143256No Hit
GGGATCACTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTT2380.1353295387992062No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCTGTCTCTTAT2370.13476092729164654No Hit
GTATAAGAGACAGTGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTA2340.13305509276896746No Hit
GTGTATAAGAGACAGCGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCT2310.13134925824628838No Hit
GTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCG2300.1307806467387287No Hit
GGTATCAACGCAGAGTACATGGGGATCCGGAGGGATCACTTCAATTTGTG2270.12907481221604963No Hit
TTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATTGAAGTG2260.12850620070848992No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2240.12736897769337058No Hit
ATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCA2240.12736897769337058No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2230.12680036618581086No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATCTGTCTCTTATAC2210.1256631431706915No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2090.1188398050799752Clontech SMART CDS Primer II A (100% over 21bp)
GAGGGATCACTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGG2060.11713397055729613No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT2040.11599674754217676Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT2020.11485952452705737No Hit
TGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACA1990.1131536900043783No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATCTG1980.11258507849681862No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1970.11201646698925892No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCTG1950.11087924397413956No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACCTGTCTCTTA1900.10803618643634111No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAAAAAAA1870.10633035191366202No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT1860.10576174040610234No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACCTGTCTCTTATACACATCTCCG1850.10519312889854264No Hit
CCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCTGT1840.10462451739098295No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCTG1830.10405590588342328No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCCT1790.10178145985318451No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1780.10121284834562483No Hit
GAGTACATGGGGATCCGGAGGGATCACTTCAATTTGTGTTCTGGTGCTAT1760.10007562533050544No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1760.10007562533050544No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)