FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004047457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1394985
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG1213568.69944838116539No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA338862.4291300623304193No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC163591.1727007817288357No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG134010.9606554909192572No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA130580.93606741291125No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115140.8253852191959054No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101410.7269612218052524No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT98740.7078212310526637No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA96020.6883228135069552No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC93550.6706165299268452No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT90080.6457417104843421No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC85570.6134116137449507No Hit
GCAGAAAACAGAGAGGAATGAGAAGAAGCAGCAGATGGGCAAAGAGTACC78230.5607945605149876No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG61780.4428721455786263No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC59910.4294669835159518No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC56920.40803306128739736No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG56630.4059541858873034No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC43640.31283490503482114No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA43150.30932232246224867No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38090.27304953099854123Clontech SMART CDS Primer II A (100% over 21bp)
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT38000.27240436277092583No Hit
ATTTTACCACGACTGTTCCTGCGCTCCTTTTGCCTATACATATTCAGGCG37520.2689634655569773No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG37120.26609605121202023No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC34830.24968010408714073No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC34060.24416033147309826No Hit
CCTCTGCCCATGGCGGGGTGGAGACCCGGAACCAGCAGCCCCCTCCACTG33680.241436287845389No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG33300.23871224421767975No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG31890.22860460865170595No Hit
CAAGTGGAGACAGGAAGGGGACTAGGGGTTGGCCGGCGGGAGGGGAGGCA29630.21240371760269824No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG29550.21183023473370682No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA27150.19462574866396412No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA27090.19419563651222055No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACG26240.1881023810291867No Hit
AATTACCACTGCCCAGCCCCAGCCTCCATCTGGGGGGGTGTATCCAACTG25190.18057541837367427No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA24530.17584418470449503No Hit
GCTGCAGGATGGGGAATTTAGCATGGATCTTCGTACCAAGAGCACCGGTG24140.17304845571816183No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA23400.1677437391799912No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG22720.16286913479356407No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21760.15598734036566703No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC21600.15484037462768416No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC21580.1546970039104363No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG21090.15118442133786386No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG20980.15039588239300064No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA19840.14222375150987288No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC18420.13204443058527512No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT18410.1319727452266512No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC18410.1319727452266512No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA18410.1319727452266512No Hit
AAGTAACACTGGCAACTTTGAAAATGTCTGTACAGCCAACGGTTTCCCTT17610.12623791653673697No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC17410.12480420936425839No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA17270.12380061434352341No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG17180.12315544611590805No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC16990.12179342430205342No Hit
TGCTTCCGGATAACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA16490.11820915637085704No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG15980.11455320308103671No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG15790.11319118126718208No Hit
GTAAGACCTAAAGTTCTTATGAGATTGTCCAAAACAAAGAAACATGTCAG15480.11096893514984031No Hit
GTTTTGAATGTGTTGGGGGCAGTCACATCAAATACCAGAACGCAGCAGTC15410.11046713763947283No Hit
GGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCA14520.10408714072194325No Hit
GCCAAGACTTCCTCATCTTCCTTTGAAGAAACGAATGAAATAGCCAAACC14400.1032269164184561No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCT14080.10093298494249041No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT14020.10050287279074685No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)