FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004047465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2286631
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA1788967.823562262560071No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA447031.954972183968467No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG178840.782111324476927No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA161830.7077224090813078No Hit
GTACTTGTTTGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATT129930.5682158599266782No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC121300.530474746471993No Hit
GTATCAACGCAGAGTACATGGGGCGACCCCAGGTCAGGCGGGACTACCCG100830.4409543997260599No Hit
GAATTTACCGCCCGATTGGGGCTGCATTCCCAAACAACCCGACTCGCCGA95150.4161143621336368No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTAAGCATCCGCA92400.4040879354823756No Hit
GTTGTGCTCCCCAGAGCGTCTGGGCTTACCACCAGCACCCCAAGGAGAAG87430.3823529025890054No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG83650.36582203250109No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82320.3600056152479346No Hit
TATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAA82220.35956829064243423No Hit
CGGTACTTGTTTGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAA82070.35891230373418365No Hit
GTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAA81970.3584749791286832No Hit
GTACATGGGGCGACCCCAGGTCAGGCGGGACTACCCGCTGAGTTTAAGCA78180.3419003765802178No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67290.29427572704122357No Hit
GTCTCTCGCCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGC61380.2684298428561495No Hit
CTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATTTACCGCCCGAT55970.24477058169857752No Hit
CCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCC54330.23759845816837083No Hit
CTCCAACTGACTGTTGTCAGTAAATGTCCCAAAGTCTGTTCCTATGAGAT48820.21350187240529844No Hit
TTCCCAAACAACCCGACTCGCCGACAGCGCCTCGTGGTGCGACAGGGTCC47730.20873503420534403No Hit
GTGCGACAGGGTCCGGGCACGACGGGGCTCTCACCCTCTCCGGCGCCCCC45760.20011973947698602No Hit
GCGTAAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGT44580.1949593091320812No Hit
GCTCTCACCCTCTCCGGCGCCCCCTTCCAGGGGACTTGGGCCCGGTCCGC43760.1913732473669779No Hit
GTACATGGGCAGAAATCACATTGCGTAAGCATCCGCAGGGACCATCGCAA43120.18857436989177528No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC40940.1790406934918664No Hit
TCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTG40260.17606688617446364No Hit
GCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAACAACCC39810.17409892544971184No Hit
CCCCAGGTCAGGCGGGACTACCCGCTGAGTTTAAGCATATCAATAAGCGG39710.17366160084421142No Hit
GGTATCAACGCAGAGTACATGGGGCGACCCCAGGTCAGGCGGGACTACCC39130.17112511813230907No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTTACAAGGATTCCC39110.17103765321120898No Hit
GTATCAACGCAGAGTACATGGGCGCGACCCCAGGTCAGGCGGGACTACCC37410.16360313491770206No Hit
GTACATGGGTGATCATTGGTCCGATGCTTCGGGCGAAACCAATTCCCAGG36860.16119784958744984No Hit
GGACTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTT35320.15446305066274357No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTAAGCATCCGC35170.15380706375449296No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACA34460.15070205905544007No Hit
GTTTGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATTTACCGC34150.1493463527783888No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA34110.14917142293618865No Hit
GCTTGAGAATCGGGCGCCTTCACGGGCGTCTCCGAATTGTAGTCTGGAGA33670.14724719467198688No Hit
GGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAACAACCCGACTC31970.13981267637847994No Hit
GTACATGGGCGCGACCCCAGGTCAGGCGGGACTACCCGCTGAGTTTAAGC31150.13622661461337662No Hit
GAATTGTAGTCTGGAGAAGCGTCCTCAGCGGCGGACCGGGCCCAAGTCCC30840.13487090833632537No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30630.13395252666477453Clontech SMART CDS Primer II A (100% over 21bp)
ACTTGTTTGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATTTA29840.13049766228132129No Hit
GGGCTGCATTCCCAAACAACCCGACTCGCCGACAGCGCCTCGTGGTGCGA29740.13006033767582087No Hit
ACCGGGAACAGCCCAGCTTGAGAATCGGGCGCCTTCACGGGCGTCTCCGA29580.12936061830702023No Hit
CTTCCAGGGGACTTGGGCCCGGTCCGCCGCTGAGGACGCTTCTCCAGACT29060.12708653035841813No Hit
ATACAGTCTCCTTCCAGAGGTCGGGGGTCAGGTAGCTGTAGGTCTTAGAA28800.12594948638411707No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG28670.12538096439696653No Hit
GTATCAACGCAGAGTACATGGGTGATCATTGGTCCGATGCTTCGGGCGAA28590.1250311047125662No Hit
GGGTGAGAGCCCCGTCGTGCCCGGACCCTGTCGCACCACGAGGCGCTGTC28280.12367539843551496No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTTACAAGGATTCCCCT28110.12293194660616426No Hit
GAACAGCCCAGCTTGAGAATCGGGCGCCTTCACGGGCGTCTCCGAATTGT28020.1225383544612139No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG26610.11637207752365818No Hit
CTCCGGCGCCCCCTTCCAGGGGACTTGGGCCCGGTCCGCCGCTGAGGACG26600.11632834506310813No Hit
CGCCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATT26450.11567235815485753No Hit
CTGTCGCACCACGAGGCGCTGTCGGCGAGTCGGGTTGTTTGGGAATGCAG26120.11422918695670617No Hit
GTCCGGGCACGACGGGGCTCTCACCCTCTCCGGCGCCCCCTTCCAGGGGA25670.11226122623195435No Hit
GGATTCCCCTAGTAACGGCGAGCGAACCGGGAACAGCCCAGCTTGAGAAT25630.11208629638975419No Hit
CCCCTAGTAACGGCGAGCGAACCGGGAACAGCCCAGCTTGAGAATCGGGC25500.11151777440260364No Hit
CTACAATTCGGAGACGCCCGTGAAGGCGCCCGATTCTCAAGCTGGGCTGT25320.11073059011270292No Hit
GATTGGGGCTGCATTCCCAAACAACCCGACTCGCCGACAGCGCCTCGTGG25180.11011833566500234No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC24590.10753812049254996No Hit
GTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGA24300.10626987913659879No Hit
GGTCAGGCGGGACTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAA24240.10600748437329854No Hit
GTCCCGAACCCGTCGGCTGTCGGCGGACTGCTCGAGCTGCTCCCGCGGCG23770.10395205872744662No Hit
GGGTCCGGGCACGACGGGGCTCTCACCCTCTCCGGCGCCCCCTTCCAGGG23300.10189663308159472No Hit
GTCCGCCGCTGAGGACGCTTCTCCAGACTACAATTCGGAGACGCCCGTGA23190.10141557601554425No Hit
TCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCA22980.10049719434399342No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA22970.10045346188344337No Hit
CGCCGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTCTC22870.10001613727794295No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)