FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004047470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1043922
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC829677.947624439373822No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA267582.563218324740737No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC136961.3119754157877697No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA92050.8817708602750014No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG90990.8716168449366907No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78460.7515887202300555No Hit
GTATAGAAGTGGTGTAACGACGGCTACCGCAAAAATAGCATAAAATGGAT77210.7396146455386513No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT76100.7289816672126845No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA75030.7187318592768426No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT74050.7093441847187817No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC72310.6926762727483471No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC70830.6784989683137246No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70550.67581677558285No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG56580.5419945168317173No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC46920.449458867616546No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG44090.42234956251520706No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC43030.4121955471768963No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC34890.3342203727864726No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC33600.32186312770494346No Hit
TGATGAAGGTGGGACACCTCTCGGAGCCACTAATGAACCGCCTGGAAGAC32870.31487026808516344No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT31440.3011719266381971No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG29800.28546194064307484No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC26560.25442513904295533No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26530.2541377612503616Clontech SMART CDS Primer II A (100% over 21bp)
GACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTC26130.25030605734911227No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA24990.2393857012305517No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC24820.23775722707252075No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG24650.23612875291448976No Hit
CCCTGTGGCTTGTTCAACGTCACTAGAGGTCCGGGCTTCGGTGCCAAAGC22460.2151501740551497No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG22210.2127553591168689No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA21110.20221817338843323No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA19770.18938196531924797No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG19750.18919038012418554No Hit
CTGAAAACGTTTTAGGAAAAAGCTGACCAAGAAGACCTGAACTAAAGTAG19630.18804086895381072No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA19260.1844965428451551No Hit
AGTCAGATACCCGTGACAATCCCGTGTAGAAGGCCATCATGAGCAAAGTG18970.18171855751674934No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA17940.1718519199710323No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC17350.16620015671668956No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG15820.1515438892944109No Hit
CTTCAGGTTCTCCGGCCTGCCTTCACTGGTTTGTGTCCAGAGCCGGACTG15750.15087334111169226No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15380.14732901500303663No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA14780.14158145915116263No Hit
GTATCAACGCAGAGTACATGGGGATCCGGAGGGATCACTTCAATTTGTGT14550.13937822940794428No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC14340.1373665848597884No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC14330.13727079226225714No Hit
AACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATT14300.13698341446966344No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG13550.12979896965482096No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG13270.12711677692394643No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA12520.11993233210910394No Hit
TGCTTCCGGATAACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA12160.11648379859797954No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT12110.11600483561032338No Hit
ATTTTCATATTGAATTGCAAATTCGAAGAAGCAGCTTCAAACCTGCCGGG11890.11389739846463624No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG11720.11226892430660527No Hit
GGGCGCCGCCGATGTCGTCGATGCTCTTCACCGCGTCGCCCACGGCGGCC11690.11198154651401158No Hit
GGTCACCACTGTCCAGCCTCACGCCCACTGCCCGGTAGCCCAGCTCTCCC11460.10977831677079321No Hit
GAACAGACACGGGTGAATGCTGCTAAAAACAAGACTGGGGCTGCTCCCAT11430.10949093897819952No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG11380.10901197599054335No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT11240.1076708796251061No Hit
GGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCA11160.10690453884485622No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC11150.10680874624732499No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA11030.10565923507695019No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10510.10067802000532607No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)