FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004047475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences647978
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC211133.2582896332900195No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC126341.9497575534971865No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA108791.6789150248928204No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA74521.1500390445354625No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC68721.0605298328029655No Hit
CATGGCGTTGGGGAGGTCCTGCTTGTTGGCGAACACCAGGAGGACAGCAT67941.048492387087216No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT66081.019787708841967No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT63820.984909981511718No Hit
GAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTT55440.8555846031809722No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51040.7876810632459744No Hit
ATGCCCAGCTAATTTTCGTATTGTTTGTAGAGATGGGGTTTAGCCACGTT43730.6748685912176031No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41690.6433860408841041No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC37900.5848964008037311No Hit
GTGTAGCTCTGACCGGCCCTGCGAGGAACTCACGAAGAACCTGCTCTCGG36690.5662229273216066No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG36680.5660686010944816No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC36090.5569633536941069No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA35240.5438456243884824No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC29650.4575772634256101No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC28610.4415273358046107No Hit
GAGTACATGGGGAATAACTGCAATCCCCGATCCCCATCACGAATGGGGTT27630.42640336554636116No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT27570.4254774081836112No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG26400.4074212396099868No Hit
GCTGAGGAGAAGGCTGCCGTCACTAGCCTGTGGAGCAAGATGAATGTGGA25530.3939948578501122No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG24640.38025982363598765No Hit
GTAGGAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAA23120.35680223711298836No Hit
CTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCGCGGGT22440.3463080536684887No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC20370.3143625246536148No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG20090.3100413902941149No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT19400.29939288062249025No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16940.2614286287497415Clontech SMART CDS Primer II A (100% over 21bp)
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA16910.26096565006836653No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC15720.24260082904049213No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA15500.23920565204374222No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG13980.215748065520743No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC13560.20926636398149323No Hit
GGCTACTATCACAAGCCTGTGGGGCAAGGTGAATGTGGAAGATGCTGGAG13420.20710579680174326No Hit
GGTGGATCCTGACGATCGCGTGGCGGTGGAGAACGTGACGCTGCGCATGC12890.19892650676411852No Hit
GCATCCTACTCTCTCCTCAATTCCATCTGTTAATCCACCAACCCAAATCC12850.19830920185561854No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT12170.18781501841111892No Hit
GGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCA11680.18025303328199413No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG11540.17809246610224422No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11270.17392565796986936No Hit
GGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTA11050.17053048097311946No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10790.1665179990678696No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG10600.16358580075249468No Hit
AGATGGGCAGATCACCTGAGGTCAGGAGTTCGAGAGCAGCCTGACCAACA10260.15833870903024486No Hit
GTATCAACGCAGAGTACATGGGGATCCGGAGGGATCACTTCAATTTGTGT10060.15525218448774494No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA9770.1507767239011201No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG9540.14722722067724522No Hit
TTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGTTCTTAG9450.14583828463312026No Hit
CCCCTAAACTGACCGTTCTTAAAGATAACTGGTGCTCATTTTCATCATTA9410.14522097972462028No Hit
CAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAA9030.13935658309387047No Hit
GGATTTTCACGGGCCGTCACAAGCGCACCGGAGCCAGCAAAGGTGCTGGC9010.13904793063962048No Hit
GGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAA8810.1359614060971206No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG8570.1322575766461207No Hit
CTCTAGAAGAAATTAACGAGCTATACCACGATGAAATCGATCCTGCTACG7960.12284367679149599No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC7930.12238069811012103No Hit
AACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATT7480.11543601788949626No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG7420.11451006052674627No Hit
CTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATC7260.11204084089274635No Hit
CAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTA6980.10771970653324649No Hit
GTACATGGGGATCCGGAGGGATCACTTCAATTTGTGTTCTGGTGCTATCC6940.10710240162474652No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA6930.10694807539762152No Hit
GAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGTTGATAC6680.10308991971949666No Hit
CCTGACGAGTAGGCAGGCGTGGAGGTCAGTGACGAAGCCTAGACCGTAAG6620.10216396235674667No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)