FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004047476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1069037
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA11759811.000367620578148No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA430664.028485449989103No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA146501.3703922315130346No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG128151.1987424195794907No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC126941.1874238216263795No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC110071.0296182452057319No Hit
CCAAACATCATTTCAGGGAGAGATGGCATATGTAATTCATCGGCTAATTT76230.7130716710459974No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70580.6602203665541978No Hit
CAGGTGACTACACCCTGGACAGATTCCAGTCAGCGCCCAACCCCAGCCCC70280.657414102598881No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT66130.6185941178836654No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66040.6177522386970703No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA65400.6117655422590612No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC61660.5767807849494451No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT61410.5744422316533478No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG60750.5682684509516509No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG60200.5631236337002368No Hit
GGTGAAAGCGTGCCTGGTTTGTTTAAGGAATACTGAGGAGGCTAGTGTGG56800.5313193088733131No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA39180.36649807256437333No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC38890.36378535074090046No Hit
GTGACTGTGGAGCCCATGGACCAGTTAGATGATGAAGAGGGACTTCCAGA37970.35517947461126226No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC36560.34199003402127337No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC32860.3073794452390329No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC32350.30260879651499434No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC31580.29540605236301454No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA28050.26238567982212024No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27260.2549958514064527Clontech SMART CDS Primer II A (100% over 21bp)
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA27000.2525637559785115No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG26000.24320954279412219No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT25820.2415257844209321No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACA24160.22599779053484584No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT23440.21926275704208553No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC22700.21234063928563746No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA21470.20083495706883858No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG20830.1948482606308294No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG20470.19148074388444927No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC20060.18764551647884967No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG19440.18184590430452827No Hit
GCTTGGACACGTTGAGAATCTGCTGTGCCTCCTGGAGGCTGAGGCCGGAG19240.17997506166765043No Hit
TGCTTCCGGATAACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA18580.17380128096595346No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG16720.15640244444298934No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG16390.15331555409214087No Hit
CAAACTGCCTGGGCAGAGTTCCCCGACTAGGCTGATAAAATGGCTTTGAA15600.14592572567647333No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA14920.1395648607110886No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG14860.13900360792002522No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA14820.13862943939264963No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC14710.13760047594236682No Hit
GTATCAACGCAGAGTACATGGGGATCCGGAGGGATCACTTCAATTTGTGT14680.13731984954683515No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14090.13180086376804545No Hit
GTATGAGGCGGTAGCGGACCGCGGAGTCGAGACCTACCCGAACGACGCGG13690.12805917849428972No Hit
CATCACCACGAACTCGAGGGTTGTCTGAATCATAGCCACGATGTGTCGCC13620.12740438357138245No Hit
AGGCTACTAGGTCCACACCCAGTGTCTTGGAAACGAAGGGGAAGGAGCGA13550.12674958864847521No Hit
CCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCGC13250.12394332469315841No Hit
AACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATT12960.12123060286968552No Hit
CGTCAGATGTGTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCAC12790.11964038662833933No Hit
TTACTTGAGCTCTGTGCTTTGAAACCAGTTTGATAAGTCCTTTACTAAGG12700.1187985074417443No Hit
CGTGGTTTTTCTGCCTCAGCCTCCCGAACCAGAGCTGTCTCTTATACACA12610.11795662825514926No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG12060.11281181100373514No Hit
CCTTAATCGCCGTCTCAAACATTTCGGGCTTTTTAATGATGAACTTAATT11760.11000554704841835No Hit
CTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATC11220.10495427192884811No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACCTGTCTCT11040.10327051355565804No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG10840.10139967091878016No Hit
GCATGAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACA10760.10065133386402902No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT10710.10018362320480957No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)