FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004047477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1436839
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC588414.09517002252862No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA187271.3033471391018756No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC161131.1214200059992803No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102960.7165729772089984No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA95240.6628439233623252No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT95010.6612431873021264No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92940.6468365627603372No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT90150.6274189383779254No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC84470.5878877174130157No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA66490.46275191583747377No Hit
CTCAGCACATCCTTGGTATGGCCCACAAATCGCCTCGTGGTGGTGCCCGT64940.451964346736134No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA58630.4080485009106796No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG55490.3861949738279654No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC53870.37492022418656507No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC53140.3698396271259341No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC50150.34903005834334955No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG45730.3182680870995289No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC43300.30135596263742837No Hit
GGTCAGCTTTGCTCCTAACAACACTGGGAAAGAAGTAACAGTTGAGTGCA39300.27351707463397085No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT35890.24978442261102324No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA35570.24755731157074662No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34420.23955363126975257Clontech SMART CDS Primer II A (100% over 21bp)
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG34370.23920564516970935No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG31260.21756090974702105No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG28790.20037039640488602No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG26340.18331907750276824No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC25930.1804655914824138No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC25760.17928243874226688No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG25520.1776121054620594No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC24360.16953882794105674No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG22880.1592384393797774No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21100.14685013421823878No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG20850.1451102037180227No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC20410.14204792603764235No Hit
CCCCTGCCCCTGGCACCCAGCTGTGGAGGGGACACTGTCTTTGAGGCATC20330.1414911482775732No Hit
TCCCCTGCCCCTGGCACCCAGCTGTGGAGGGGACACTGTCTTTGAGGCAT19590.13634095399693355No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA19410.13508820403677796No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA19260.13404424573664828No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG18640.12972921809611238No Hit
GCACTAGTCTGATGACCTGCTAATTCACTGACAGCATAGGGCTGTCTGTT17950.12492700991551593No Hit
ACTGTGACTTCTGCCTGGGGGGCTCCAAGAAGACGGGGTGTCCCGAGGAC17940.12485741269550729No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC17070.11880245455475527No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC17010.1183848712347034No Hit
CTGCTGTTGGTTGGGGGCCGTCCCGCTCCTAAGGCAGGAAGATGGTGGCC16790.11685373239451323No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA16550.11518339911430577No Hit
GTGGCAGTACAGACTTATAGTCCTAGCAACTTGAGAGGCTAAGGCGGGAG15830.11017239927368341No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG15520.10801488545341545No Hit
GAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTT15400.10717971881331172No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT14700.10230791341270665No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG14690.102238316192698No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)