FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004047480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1113131
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG997528.961389090771887No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA268522.412294689483987No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC141601.272087472184316No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG91770.82443126640081No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT87600.7869693683852125No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA86240.7747515791043462No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA85540.7684630110921356No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT84370.757952118843155No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84020.7548078348370497No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC78400.7043196173675875No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77270.6941680718621618No Hit
ACCACTTTCTCTGCCACCAAGTAGGCAGAATAAAAGCCAACACCAAACTG68920.6191544391450782No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC62530.5617487968621843No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG55830.5015582173167399No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC48870.43903188393818876No Hit
GAGAAAGAGCTATCAATCTGTCAATCCTGTCCGTGTCCGGGCCGGGTGAG47240.42438850413832696No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC46500.4177405893825615No Hit
GAGGGGCAGCTATATGCCATGGAGTTACAGGGCTTCTGGATGGACATTGG42630.3829737919436257No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG41140.369588125746206No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC40840.3668930251695443No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA39990.35925690686900286No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT35460.31856088816141137No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC33060.29700008354811785No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG32740.29412530959967875No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC31000.278493726255041No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG29680.26663528371772954No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27030.24282856195721797Clontech SMART CDS Primer II A (100% over 21bp)
TAAGTACCACTGAGTTCTCTTCCAGTTTGCAGGTGTTCCTGTGACCCTGA26740.24022329806644502No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT24670.22162710408747938No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA24220.21758445322248685No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA22770.204558133768622No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC22760.20446829708273329No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA22560.2026715633649588No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG22140.19889842255763246No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA22040.19800005569874526No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG20910.18784851019331958No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACG20820.18703998002032104No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19690.1768884345148954No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC18930.17006084638735244No Hit
CCTGATGCCTCTAAGCTGCAATTCATCCTAGCAGAGGAGCAGTTTATCCT18520.16637754226591478No Hit
GCAGAGTACATGGGGACCCGAGGGATCACTTCAATTTGTGTTCTGGTGCT17400.15631583344637784No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG17180.15433942635682593No Hit
GTATATGAATGAGAATGACAGTCCTGGTCTCTGGCCTCAGAGAGCTCACA16750.15047644886361083No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA16590.14903906188939128No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG16270.14616428794095215No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC16130.14490657433851004No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA16060.14427771753728896No Hit
GGTGAATTCTTTGCCGAAATGGATTGCCAAAACGGTCACCAGCACATTTC15240.1369111092944137No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG14680.1318802548846452No Hit
GGTTTGAAGTGCTTGGCCACCTCCAGGTAATCATCTAAGAGACCTAGGCT14600.13116156139753543No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG13540.12163887269333079No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC13370.12011164903322251No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT13220.11876409874489167No Hit
AGAGACTTCCTCCACTGCCTCTGCCTGTCTCTTATACACATCTCCGAGCC13060.11732671177067208No Hit
GTCACTGTGCAGGCGCTTGGGTGACGCGACGATCTCAGCGGATCTGGTCA13050.11723687508478338No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA13050.11723687508478338No Hit
TGCTTCCGGATAACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA13010.11687752834122848No Hit
AGCCTTACCCTTGATAATGGCGTATGGAACACCCATCTTCTTACACAAAG12530.11256536741856979No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA12450.11184667393146No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAG11970.10753451300880129No Hit
GGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCA11830.10627679940635917No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC11730.10537843254747195No Hit
GTATCAACGCAGAGTACATGGGGATCCGGAGGGATCACTTCAATTTGTGT11650.10465973906036218No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG11570.1039410455732524No Hit
GTCTGATGTCATGCGCTTTCTTCTGAGGGTCCGCTGCTGGCAGTACCGCC11420.10259349528492154No Hit
AACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATT11330.10178496511192303No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)