FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004047487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1038038
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA12100311.656895026964332No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA474374.569871237854491No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA158711.528942100385535No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG130051.2528443082045166No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC126821.2217279136216592No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC115971.1172038017876031No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG98120.9452447790928655No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT67010.6455447681106087No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA65070.6268556642435056No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62690.6039277945508739No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62300.6001707066600644No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG59830.5763758166849383No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC59180.5701140035335893No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT57450.5534479469923067No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA49770.47946221621944474No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC48840.4705030066336685No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC42660.4109676139023812No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA41130.39622826909997516No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC36950.35595999375745396No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC34090.3284080158915184No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC32700.31501736930632596No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA30290.2918004928528628No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT30150.29045179463564913No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA29620.28534600852762615No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT29030.27966220889794013No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC27810.26790926729079284No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACA25540.24604108905454328No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25310.2438253705548352Clontech SMART CDS Primer II A (100% over 21bp)
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG24180.23293944923018234No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC23320.2246545887530129No Hit
AACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATT22000.21193829127642724No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG20300.1955612414959761No Hit
GTATCAACGCAGAGTACATGGGGATCCGGAGGGATCACTTCAATTTGTGT19200.1849643269321547No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG18530.17850984260691805No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG18280.17610145293332227No Hit
CGTCAGATGTGTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCAC17730.1708029956514116No Hit
TGCTTCCGGATAACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA17730.1708029956514116No Hit
CTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATC17290.16656422982588306No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC16350.15750868465316298No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG16340.15741234906621915No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA16030.1544259458709604No Hit
ACAGGGGTGCTGGCGCAGCCCTGGGGCGGGTGCCCGTTGAAGCTGTCTGT15930.1534625900015221No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACCTGTCTCT15090.14537040069824034No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14350.13824156726439688No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA14310.13785622491662156No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG13610.1311127338305534No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG13520.13024571354805894No Hit
GTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCG12580.12119016837533886No Hit
GTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTC12540.12080482602756354No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCCTGTCTCT12180.11733674489758564No Hit
CCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGA12010.11569903991954052No Hit
GCATGAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACA11860.11425400611538306No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGC11370.10953356235513535No Hit
GTACATGGGGATCCGGAGGGATCACTTCAATTTGTGTTCTGGTGCTATCC11350.1093408911812477No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG11300.10885921324652854No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCTGTCTCTTATACAC11270.10857020648569705No Hit
ACACTACCTTGAACTCGGCATCAGAGGGAGACCTGTCTCTTATACACATC11170.10760685061625874No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT10870.10471678300794382No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG10800.10404243389933701No Hit
GGAGGTTACCGAAGAGCAAGCTGAAGCTACTGAATGGGCTGAAGAAAATG10630.10240472892129189No Hit
ATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATTGAAGT10590.10201938657351659No Hit
TACCACCTCTTCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACT10560.10173037981268508No Hit
GGTATCAACGCAGAGTACATGGGGATCCGGAGGGATCACTTCAATTTGTG10550.10163404422574125No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)