FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004047502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences557736
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT7921214.202418348465942No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA451598.096841516416369No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA159642.8622860995166173No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC121542.17916720455556No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG102571.839042127458152No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA95861.7187343115739346No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA56271.0089002682272616No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT53860.9656898604357617No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC49300.8839307485979029No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG48960.8778346744696415No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA47430.8504023408924652No Hit
CGTCAGATGTGTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCAC41600.7458725992225712No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC40870.7327839694765983No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC37790.6775607097264655No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC33690.6040492275915488No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA32710.5864781903983246No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACCTGTCTCT32640.5852231163130944No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC30600.548646671543526No Hit
CTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATC28190.505436263752026No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24520.4396345224263809No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24370.43694507795803034No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT23520.42170489263737687No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCTG23480.4209877074458167No Hit
GTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTC22800.40879555918929383No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA22410.4018030035715822No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCCTGTCTCT22350.400727225784242No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTCTG21140.379032373739547No Hit
GTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCG20560.36863318846192467No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT18790.33689774373538733No Hit
GCGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTAT18490.33151885479868615No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCTGTCTCTTATACAC17900.3209403732231737No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG16390.29386663224177745No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC16270.29171507666709695No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACCTGTCTCTTATACACATCTCCG16150.2895635210924165No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCTGTCTCTTAT14930.26768937274983146No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC14910.2673307801540514No Hit
AACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATT14710.26374485419625054No Hit
GTGTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCACCTGTCTCT14570.26123470602578996No Hit
GTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTA14420.2585452615574394No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAA13750.24653240959880662No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTCTGTCTCT12820.22985785389503277No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATCTGTCTCT12730.22824418721402243No Hit
CGTCAGATGTGTATAAGAGACAGTGCTTCCGGATGACTGACGACTCTCAC12300.22053444640475062No Hit
CCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGA11980.21479696487226932No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCCTGTCTCTTATACACAT11890.21318329819125897No Hit
GATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACAT11780.21121103891446846No Hit
CGTCAGATGTGTATAAGAGACAGCGTGGTTTTTCTGCCTCAGCCTCTGGA11570.20744581665877765No Hit
GCAATCGCAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGC11170.200273964743176No Hit
CGTGGTTTTTCTGCCACAGCCTCTGGAGCCTGTCTCTTATACACATCTCC11150.1999153721473959No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT11100.19901889065794567No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCTG10990.19704663138115525No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT10940.19615014989170504No Hit
GTGTATAAGAGACAGGCTCCAGAGGCTGTCTCTTATACACATCTCCGAGC10870.19489507580647475No Hit
GCAGCGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCT10830.19417789061491458No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC10790.19346070542335442No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACCTGTCTCTTA10330.1852130757204125No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACG10060.18037207567738142No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACCTGTCTCT9980.1789377052942611No Hit
CGTGGTTTTTCTGCCTCCCGAACCAGAGCTGTCTCTTATACACATCTCCG9580.17176585337865943No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATCTGTCTC9510.17051077929342914No Hit
GGCTCGTGCCCTGGCTGGAAGAGCCAGGTCAGGGAGAAGGGGTCTGGTCA9490.17015218669764906No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGCTGTCTC9390.16835922371874865No Hit
GCATGAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT9350.1676420385271885No Hit
TGCTTCCGGATAACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA9090.16298033478204743No Hit
CGTGGTTTTTCTGCCTCAGCCTCCCGAACCAGAGCTGTCTCTTATACACA8380.15025029763185452No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCT8230.14756085316350387No Hit
TCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCAC8070.1446921123972632No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGCT7950.14254055682258274No Hit
GATCACTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCG7840.14056829754579225No Hit
ACTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAAT7660.13734096418377154No Hit
TACCACCTCTTCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCG7600.1362651863964313No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACCTGTCT7570.13572729750276116No Hit
GCGTCAGATGTGTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCA7360.1319620752470703No Hit
GATGTGTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCACCTGTC7190.12891403818293962No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG7060.12658318631036905No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCCTGTC7020.1258660011188089No Hit
CCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACC6850.12281796405467821No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCTG6840.12263866775678817No Hit
GTATCAACGCAGAGTACATGGGGATCCGGAGGGATCACTTCAATTTGTGT6740.12084570477788774No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGCTGTCTCTTATAC6680.11976992699054749No Hit
ACACTACCTTGAACTCGGCATCAGAGGGAGACCTGTCTCTTATACACATC6630.1188734455010973No Hit
GGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACA6570.11779766771375705No Hit
GCGAAGGCTTCCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTC6540.11725977882008692No Hit
GTATAAGAGACAGCGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCT6500.11654259362852676No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT6370.11421174175595622No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA6240.1118808898833857No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG6230.11170159358549565No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATCTG6220.11152229728760561No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6200.11116370469182553No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6150.11026722320237531Clontech SMART CDS Primer II A (100% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG6050.10847426022347491No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCCTGTCTCT5870.10524692686145418No Hit
GGCAGCGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTC5800.10399185277622387No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCTGTCTCT5610.1005852231163131No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)