FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004047503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428349
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG263106.142187795465847No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA201554.7052753712510125No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG153473.582826153440302No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA71711.674102192371174No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA66921.5622774886832933No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA65771.5354302216183533No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC59401.3867197075282072No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC54841.2802644572533144No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA43561.0169277855206853No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT41640.9721045222470461No Hit
CGTCAGATGTGTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCAC36100.8427707313429003No Hit
CTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATC34790.8121881923384904No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC31810.7426187524658632No Hit
AACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATT27580.6438675005661272No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC24040.5612246089053552No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACCTGTCTCT21900.5112653467149451No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT20870.48721953360460746No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG19090.44566463327800465No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19080.4454311787817878No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCCTGTCTCT18430.4302566365276912No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18370.42885590955039No Hit
CCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGA17260.4029424604703174No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC17030.3975730070573294No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC16930.3952384620951608No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCTG16690.38963555418595586No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA16340.3814646468183654No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTCTG16200.3781962838713292No Hit
GTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTC14940.3487810173480036No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC14730.34387847292744933No Hit
GTATCAACGCAGAGTACATGGGGATCCGGAGGGATCACTTCAATTTGTGT13500.3151635698927744No Hit
GCGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTAT13460.31422975190790686No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCTGTCTCTTATACAC13440.31376284291547313No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA13240.30909375299113573No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC12970.30279048159328026No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCTGTCTCTTAT12400.28948357530891866No Hit
GTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCG11570.27010685212291846No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT11150.26030176328180993No Hit
GTGTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCACCTGTCTCT10280.23999122211094226No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC10270.23975776761472536No Hit
ATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATTGAAGT10270.23975776761472536No Hit
TCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCAC10100.23578904117903857No Hit
GTACATGGGGATCCGGAGGGATCACTTCAATTTGTGTTCTGGTGCTATCC9890.23088649675848433No Hit
CGTCAGATGTGTATAAGAGACAGTGCTTCCGGATGACTGACGACTCTCAC9700.2264508613303638No Hit
GGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACA9630.22481667985684567No Hit
ACTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAAT9560.2231824983833276No Hit
GATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACAT9520.22224868039846013No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT9500.2217817714060264No Hit
GATCACTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCG9410.21968068094007456No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCTG9270.2164123179930384No Hit
CGTCAGATGTGTATAAGAGACAGCGTGGTTTTTCTGCCTCAGCCTCTGGA9060.2115097735724841No Hit
GCAGCGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCT9010.21034250109139974No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCCTGTCTCTTATACACAT8990.20987559209896603No Hit
GGTATCAACGCAGAGTACATGGGGATCCGGAGGGATCACTTCAATTTGTG8910.20800795612923106No Hit
GTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTA8010.1869970514697128No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAA7660.17882614410212233No Hit
CCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACC7440.1736901451853512No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG7320.17088869123074876No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACCTGTCTCTTATACACATCTCCG6830.15944942091612213No Hit
GCGTCAGATGTGTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCA6700.15641451246530283No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC6660.15548069448043533No Hit
TTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATTGAAGTG6630.15478033099178473No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACG6550.15291269502204977No Hit
TTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTG6310.1473097871128449No Hit
GATGTGTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCACCTGTC6220.14520869664689307No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCT6200.14474178765445933No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAGG6100.14240724269229063Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCCTGTC5960.13913887974525443No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGCT5830.13610397129443513No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG5790.13517015330956766No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG5660.13213524485874836No Hit
CGTGGTTTTTCTGCCTCAGCCTCCCGAACCAGAGCTGTCTCTTATACACA5620.13120142687388087No Hit
GTATCAACGCAGAGTACATGGGATCACTTCAATTTGTGTTCTGGTGCTAT5580.1302676088890134No Hit
ACACTACCTTGAACTCGGCATCAGAGGGAGACCTGTCTCTTATACACATC5560.12980069989657966No Hit
CAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGT5540.12933379090414593No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATCTGTCTC5530.12910033640792906No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGCTGTCTCTTATAC5450.1272327004381941No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACCTGTCTCT5430.12676579144576036No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5390.12583197346089287Clontech SMART CDS Primer II A (100% over 21bp)
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTCTGTCTCT5310.12396433749115791No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGCTGTCTC5260.12279706501007358No Hit
GGAGGGATCACTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGG5230.12209670152142295No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATCTGTCTCT5210.12162979252898921No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACCTGTCTCTTA4940.11532652113113373No Hit
AATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTT4920.11485961213869998No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4880.1139257941538325No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC4840.11299197616896502No Hit
CAGACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCAC4840.11299197616896502No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG4800.11205815818409753Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCCT4680.1092567042294951No Hit
GAGTACATGGGGATCCGGAGGGATCACTTCAATTTGTGTTCTGGTGCTAT4670.10902324973327823No Hit
GGCAGCGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTC4630.10808943174841076No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT4620.1078559772521939Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGC4590.10715561376354328No Hit
GCAATCGCAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGC4570.10668870477110953No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAG4570.10668870477110953Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACCTGTCT4560.10645525027489268No Hit
TGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACA4550.1062217957786758No Hit
TATCAACGCAGAGTACATGGGGATCCGGAGGGATCACTTCAATTTGTGTT4480.10458761430515771No Hit
GTACAGGGATCACTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGC4420.1031868873278565No Hit
CGTGGTTTTTCTGCCACAGCCTCTGGAGCCTGTCTCTTATACACATCTCC4300.10038543337325405No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)