FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004047505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1116036
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC1070419.591178062356413No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA369973.315036432516514No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC133061.1922554469569082No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA121291.0867928991537907No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG119091.0670802733961986No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC92790.8314247927486209No Hit
CCTTTCCATCCAGTCCTCGTACTGCATCTTCTGCATCTCTAGGATCTTCG81430.7296359615639638No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76000.6809816170804526No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69880.6261446763366056No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA68250.611539412707117No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT67200.6021311140500845No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT65390.5859129992222473No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC59650.5344809665638026No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG58790.5267751219494712No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG58320.5225637882648947No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC41760.37418147801683815No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC37550.3364586805443552No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC33690.3018719826242164No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC33630.3013343655581003No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC31750.2844890308197943No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT28080.2516047869423567No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27390.245422190682021Clontech SMART CDS Primer II A (100% over 21bp)
GGCCAGCCCAGCTTGTATGGGTTCTGGCTACACCAGAGCACCAAGAGCCC27350.24506377930461026No Hit
CTCCATTTCTGTCAGCTCAATTTGAAGAGCCAGCATCTCTGAAGACATGC26310.23574508349193035No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA26090.23377382091617116No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA26000.23296739531699695No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA25780.23099613274123773No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG25680.23010010429771083No Hit
GAGCTGAAATCACACCACTGCACTCCAGCCTGGGCACTAGAGTGAGAACC25230.22606797630183975No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA24710.2214086283954998No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC23240.2082370102756542No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG22990.20599693916683692No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG21140.18942041296158904No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG20650.1850298735883072No Hit
GGTAAAGTGGATATTGTTGCCATCAATGACCCCTTCATTGACCTCAACTA19660.17615919199739077No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC19120.17132063840234543No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG18140.16253955965578173No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG16630.14900953015852536No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG16420.14712787042711883No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15160.13583791203867976No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA15110.1353898978169163No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA14620.13099935844363444No Hit
AACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATT14420.1292073015565806No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT14380.12884889017916987No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC14050.12589199631553102No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA13930.12481676218329876No Hit
GTATCAACGCAGAGTACATGGGGATCCGGAGGGATCACTTCAATTTGTGT13550.12141185409789648No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA13540.12132225125354379No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG13390.11997820858825343No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC12810.11478124361579735No Hit
CGTGGTTTTTCTGCCTCAGCCTCCCGAACCAGAGCTGTCTCTTATACACA12420.11128673268604239No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGC12170.10904666157722512No Hit
TGCTTCCGGATAACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA12150.10886745588851972No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC11980.10734420753452398No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC11300.10125121411854099No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)