FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004047510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1271479
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC18307014.39819297054847No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA607654.779080110642803No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG197221.5511070178901893No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA178381.4029331196189634No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC147841.1627403991729317No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC134091.0545986209760443No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT96600.7597451471868587No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG92310.72600491238943No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79180.6227393452821478No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT75440.5933247816125945No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA74610.5867969506378006No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72590.5709099403136033No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC67490.5307991716733033No Hit
CATTCACCTTCTTCACGACCTCTGCATGGTGTCTGGTCTCCTCAACTGGG62470.4913175915606942No Hit
GCCCAGCATCTTCCCCCCTTCTTCCACCACTGAGTCATCTGCTGTCTCTT57020.44845412311174626No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG56710.4460160175669437No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC51630.4060625460585664No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC46270.36390691470327075No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA45720.3595812435753953No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC42110.3311891112633398No Hit
GTTCTATAGAGCCTGGCGTCTGGACGCCGAGGGCAGCCCCGACCCCTGTC39560.3111337269431898No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC38230.30067346767032727No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA37340.2936737452997651No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC35030.275505926562688No Hit
ATGAAAGACCGTGAAACCAACAAATCAAGAGGATTTGCTTTTGTCACCTT33380.2625289131790616No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC32620.25655162216599725No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC32000.25167541107639213No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG28950.2276875984581735No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT28410.22344057589625937No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27810.21872166193857706Clontech SMART CDS Primer II A (100% over 21bp)
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG27550.21667679922358135No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA24320.19127331241805803No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG23890.18789142408171902No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA23800.18718358698806664No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT23710.18647574989441432No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC23430.18427359004749588No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG22480.17680197628116548No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG21990.17294819654905821No Hit
ATCAACGCAGAGTACATGGGCATAGCGCTTGCGGCCGCCGCATAGTCAGG21960.1727122508511741No Hit
GCAACTCATCCTTTGCACCAGCCCCTAAACTGACCGTTCTTAAAGATAAC21590.16980225391060333No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA21500.16909441681695098No Hit
CCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTCCCGCGG19830.15596010630140175No Hit
TGCTTCCGGATAACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA18440.14502795563277096No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAG17860.14046633880701137No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG17810.1400730959772045No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA17730.13944390744951352No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG16720.1315004022874149No Hit
TACCACCTCTTCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCT16540.13008472810011018No Hit
GCATGAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC16370.12874770247876685No Hit
ACACTACCTTGAACTCGGCATCAGAGGGAGACCTGTCTCTTATACACATC16040.12615229980204157No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA15250.11993906309109312No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14610.11490555486956529No Hit
GGATCTTTTGACGTGGTTCACGTGAAAGATGCCAATGGCAACAGCTTTGC14600.11482690630360393No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC14200.11168096366514901No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG13450.10578232121804608No Hit
AACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATT12740.1001982730347886No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)