FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004047511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69188
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGGCTCCAGAGGCTGTCTCTTATACACATCTCCGAGC14902.153552639185986No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG8311.2010753309822513No Hit
GGATCAATGCGGACAAATGGCGGCCGCATAACTTCAAGCGCTTGACTTCG8151.1779499335144823No Hit
GGCCGCCGCTCGCGGCGGCGGCCGAAGTCAAGCGCTTGAAGTTATGCGGC7251.0478695727582819No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT6740.9741573683297682No Hit
GTATAAGAGACAGGCTCCAGAGGCTGTCTCTTATACACATCTCCGAGCCC6480.9365785974446437No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC5560.803607562004972No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA4940.7139966468173672No Hit
GGCCGCCGCTCGCGGCGGCGGCCGAAGTCAAGCGCTTGAAGTTATGCTGT4790.6923165866913338No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA4350.6287217436549691No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT4210.6084870208706713No Hit
CTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATC4100.5925883101115801No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT3740.5405561658090998No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC3540.5116494189743886No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG3460.5000867202405042Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA3350.48418800948141294No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC3220.4653986240388507No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT3050.4408278892293461Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTGGATCAATGCGGACAAATGGCGGCCGCATAACTTCAAGCGCTTGACT2980.43071052783719715No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC2950.42637451581199054No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT2820.40758513036942823No Hit
CTTGAAGTTATGCGGCCGCCATTTGTCCGCATTGATCCAAGCGCTTGACT2700.3902410822686015No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT2620.378678383534717No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC2420.3497716367000058No Hit
CCGCCGCTCGCGGCGGCGGCCGAAGTCAAGCGCTTGAAGTTATGCGGCCG2410.3483262993582702No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC2310.3338729259409146No Hit
GGCTCCAGAGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAG2200.31797421518182345No Hit
CCGCCGCTCGCGGCGGCGGCCGAAGTCAAGCGCTTGAAGTTATGCTGTCT2120.30641151644793896No Hit
GATGTGTATAAGAGACAGGCTCCAGAGGCTGTCTCTTATACACATCTCCG2080.3006301670809967No Hit
GAGATGTGTATAAGAGACAGGCTCCAGAGGCTGTCTCTTATACACATCTC2080.3006301670809967No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC1980.2861767936636411No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1950.2818407816384344No Hit
GGATCAATGCGGACAAATGGCGGCCGCATAACTTCCAAGCGCTTGACTTC1940.2803954442966989No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1840.26594207087934324No Hit
TTGATCCAAGCGCTTGACTTCGGCCGCCGCCGCGAGCGGCGGCCTGCTCG1800.260160721512401No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCTG1560.22547262531074755No Hit
GGCCGCCGCTCGCGGCGGCGGCCGAAGTCAAGCGCTTGAAGTTACTGTCT1420.20523790252644966No Hit
CATTGATCCAAGCGCTTGACTTCGGCCGCCGCCGCGAGCGGCGGCCTGCT1400.20234722784297854No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC1390.200901890501243No Hit
AACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATT1380.19945655315950742No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG1340.1936752037925652Clontech Universal Primer Mix Long (96% over 26bp)
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA1320.19078452910909408No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC1300.18789385442562295No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTCTG1280.18500317974215183No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG1230.177776493033474No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA1220.17633115569173843No Hit
GATCAATGCGGACAAATGGCGGCCGCATAACTTCAAGCGCTTGACTTCGG1210.1748858183500029No Hit
CATAACTTCAAGCGCTTGACTTCGGCCGCCGCCGCGAGCGGCGGCTGTCT1190.1719951436665318No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG1140.16476845695785397No Hit
ACTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAAT1130.1633231196161184No Hit
GGCCGCCGCTCGCGGCGGCGGCCGAAGTCAAGCGCTTGGAAGTTATGCGG1120.16187778227438285No Hit
GGCCGCCGCTCGCGGCGGCGGCCGAAGTCAAGCGCTTGGAAGTTATGCTG1070.15465109556570503No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA1070.15465109556570503No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG1070.15465109556570503No Hit
GTGTATAAGAGACAGAGAGGAAGCCTTCGCCTGTCTCTTATACACATCTC1040.15031508354049836No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATCTGTCTCT1000.1445337341735561No Hit
GGCCGCCGCTCGCGGCGGCGGCCGAAGTCAAGCGCTTGGATCAATGCGGC990.14308839683182056No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTCTGTCTCT960.13875238480661387No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC940.13586171012314274No Hit
TTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTG940.13586171012314274No Hit
GAAGTTATGCGGCCGCCATTTGTCCGCATTGATCCAAGCGCTTGACTTCG930.13441637278140717No Hit
CAGGCTCCAGAGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGC930.13441637278140717No Hit
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCT900.1300803607562005RNA PCR Primer, Index 29 (96% over 25bp)
CATAACTTCAAGCGCTTGACTTCGGCCGCCGCCGCGAGCGGCGGCCCTGT890.12863502341446492No Hit
GTATCAACGCAGAGTACATGGGGCGAGCAGGCCGCCGCTCGCGGCGGCGG880.12718968607272937No Hit
CCTCGAGTGGAAAAGCAGAAGACACGTTGAAAAAACATAGGAACAATCCC850.12285367404752268No Hit
CCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGA850.12285367404752268No Hit
GGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCA800.1156269873388449No Hit
GGCCGCCGCTCGCGGCGGCGGCCGAAGTCAAGCGCTTGGATCAATGCGGA800.1156269873388449No Hit
GTATCAACGCAGAGTACATGGGGATCAGGTTTTGTGGAGTTATTGTCATG790.11418164999710934No Hit
CGGAGATGTGTATAAGAGACAGGCTCCAGAGGCTGTCTCTTATACACATC790.11418164999710934No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT790.11418164999710934RNA PCR Primer, Index 29 (95% over 23bp)
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATCTGTCTC780.11273631265537377No Hit
GCCGCCGCTCGCGGCGGCGGCCGAAGTCAAGCGCTTGAAGTTATGCGGCC770.11129097531363821No Hit
GCGCTTGAAGTTATGCGGCCGCCATTTGTCCGCATTGATCCAAGCGCTTG740.10695496328843151No Hit
TCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCAC740.10695496328843151No Hit
TGGATCAATGCGGACAAATGGCGGCCGCATAACTTCAAGCGCTTGACTTC740.10695496328843151No Hit
CAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGT720.10406428860496039No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA710.10261895126322483No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG710.10261895126322483Clontech Universal Primer Mix Long (96% over 26bp)
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT710.10261895126322483No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT700.10117361392148927No Hit
AATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTT700.10117361392148927No Hit
GCCGCCGCTCGCGGCGGCGGCCGAAGTCAAGCGCTTGAAGTTATGCTGTC700.10117361392148927No Hit
GGTATCAACGCAGAGTACATGGGGCGAGCAGGCCGCCGCTCGCGGCGGCG700.10117361392148927No Hit
GTACATGGGGCGAGCAGGCCGCCGCTCGCGGCGGCGGCCGAAGTCAAGCG700.10117361392148927No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)