FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004047512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1037437
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG18396317.732450259630223No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA548615.288128339359402No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG211952.0430156240812694No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA183131.76521562273179No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC127061.2247490691000995No Hit
CCTTAATGTGCTGGAGGCCGATGGTCTCGTTGAAGCGCTCAGGGATGAGG112081.0803547588913833No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG106081.0225199216916305No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC83500.8048681510298938No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG66000.6361832091972813No Hit
GTAGCACTCTGAGTATAGGATGGCACCACAGTAGCCGCAGCTGCTACTGG60180.5800834171135212No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA58780.566588621766912No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA56760.5471175599096619No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52510.5061512168931703No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC50490.4866801550359202No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50270.484559544338596No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACG49930.48128223689727667No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT45980.44320763574077265No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA43760.42180874597686413No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT43190.4163144364428877No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC38250.3686970871484245No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA37350.3600218615684615No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT36520.35202137575582904No Hit
GGACTATTCTGCTTGGAAGGTCAGCTCTGAGAGAGCTGAGTCAGATTGAG35610.3432497587805332No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC35250.339779668548548No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC35050.3378518406418896No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC34890.33630957831656283No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC32720.3153926455293189No Hit
CGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTC29580.2851257473947816No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA28910.27866752390747584No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAG27700.2670041650721924No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG25080.24174961949496693No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC24920.24020735716964017No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA24460.2357733529843258No Hit
GCATGAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACG23990.2312429574036785No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC22130.21331415787175512No Hit
TGCTTCCGGATAACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA20590.19846988299048518No Hit
ACCAGCTCTTCTGCTGCTTCTACCAGCAACCCAAATTCTGCTTCATTGTC20350.19615648950249506No Hit
TACCACCTCTTCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCG19800.19085496275918443No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19790.1907585713638515Clontech SMART CDS Primer II A (100% over 21bp)
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT18720.1804446920632289No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACCTGTCTCT18060.17408285997125608No Hit
CGTCAGATGTGTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCAC17190.1656968085772919No Hit
ACTACGGCTCGCGCGCTGGCCGCCCAGGGCCTTCCTGTAAGCTGCACTGC16890.16280506671730427No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCTGTCTCTTATACAC16730.16126280439197754No Hit
AACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATT16480.15885301950865452No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG16270.15682880020666315No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG16210.15625045183466563No Hit
TACCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAAT16050.15470818950933887No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG15930.15355149276534383No Hit
GTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTC15490.14931027137069527No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG14890.14352678765072No Hit
GTATCAACGCAGAGTACATGGGGATCCGGAGGGATCACTTCAATTTGTGT14310.13793608672141056No Hit
GCAATCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAA13600.13109229765277314No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCCTGTCTCT13450.12964642672277932No Hit
CTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATC13320.12839333858345134No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG13250.12771859881612088No Hit
GTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCG12920.12453768277013448No Hit
GGTATCAACGCAGAGTACATGGGTTCCACCAGGAAAAAAAAACCATGACC12850.12386294300280402No Hit
GTACTACACGACACGTACTACGTTGTAGCTCACTTCCACTATGTCCTATC12810.12347737742147233No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACCTGTCTCTTATACACATCTCCG12710.12251346346814312No Hit
ACACTACCTTGAACTCGGCATCAGAGGGAGACCTGTCTCTTATACACATC12650.1219351150961456No Hit
GCAATCGCAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGC12370.11923615602682379No Hit
CGTGGTTTTTCTGCCTCAGCCTCCCGAACCAGAGCTGTCTCTTATACACA12020.11586245719017155No Hit
CCTTCATGTTGCGTTCGGAGAGGCGCAGCATCCACAGGCGGAGGCGAAAG11830.11403102067884603No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCTGTCTCTTAT11730.11306710672551681No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG11640.11219958416752054No Hit
GCGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTAT11590.11171762719085594No Hit
TGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAG11190.10786197137753906No Hit
GCCCTCACCTGTGCAAAACTTACCACAGCCTTTGAGGAAGTATGGGGGGT11100.10699444881954279No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10990.10593414347088063No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC10920.10525940370355019No Hit
GCAATCGCAGGCACCTCTTCGCTGTCTCTTATACACATCTCCGAGCCCAC10700.10313879300622592No Hit
CGTGGTTTTTCTGCCACAGCCTCTGGAGCCTGTCTCTTATACACATCTCC10680.10294601021556009No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)