FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004047513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1059071
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC12668911.962276372405627No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA294672.782344148787003No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG133851.263843500577393No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA109051.0296760085017909No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC105630.9973835559655584No Hit
CTGTAAACCGATCCCCCATGGTTGTCAGCAGCTCTCTCAAGTAATCTTCC92090.8695356590823468No Hit
GTATCTCTCTTCAAACTTGACCTTGGCCTCCCGTCGGGCCTTGCGTTTAA86100.8129766559560218No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG66710.6298916692082023No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64970.6134621758125753No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC64080.605058584363088No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62120.5865517986990485No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT61340.5791868533837675No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG61260.578431474377072No Hit
GTTGAGGGATAGGAGGAGAATGGGGGATAGGTATATGAACATGAGGGTGT56810.5364135171296353No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT56340.5319756654652994No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA55830.5271601242976156No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA55380.5229111173849534No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC52860.49911667867404547No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC39560.3735349188109201No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC34210.3230189477381592No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA34040.3214137673489313No Hit
GACGAGGAATAGCAGGAAAACCTACTAAAAGGGGAGGGGGGAATCAAAAT33790.31905320795300784No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT33430.31565400242287817No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC33130.31282133114777005No Hit
ACTTAGACATGCATGGCTTAATCTTTGAGACAAGCATATGCTACTGGCAG32960.31121615075854214No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA31790.3001687327856206No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC31310.29563645874544764No Hit
CCCCAGTGCTGGAGGTCAGCGTGGCGGCTTTGGTAGCCCAGGTGGCGGCA31100.29365358885287196No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC29380.27741294020891893No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC28500.2691037711352685No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC28470.26882050400775775No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA26120.24663124568607772No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25340.2392663003707967Clontech SMART CDS Primer II A (100% over 21bp)
CACCATGGACCTGCGCAGCGATGACCAGGACCTGACCCGCATGATCCACA24610.23237346693470032No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT24030.226896969136158No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG22290.21046747574053107No Hit
TTCTTTGATCAGCCACACGAAACTTATACAAAGCTGCAACCCCCAGGGAT20900.19734276549919694No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC20400.19262164670735013No Hit
GTATCAACGCAGAGTACATGGGAGATGTAAGCTCAGGGTTACACAGTTCC20050.18931686355305735No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG19640.18544554614374295No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG19630.18535112376790602No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG19290.1821407629894502No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG18470.17439812817082143No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGC18080.17071565551318088No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA16800.15862959140605304No Hit
AACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATT15890.15003715520489183No Hit
CTCCTCTGTCTCCCAGCCCTGCACAGCTCCAGCCGGCAGCAGGAACAGCA15700.14824313006399006No Hit
ACCAAGATCAGGGTGTGATGGTGGCTGGGTGGGGGTGGAGGCTGTAGCCA15620.14748775105729456No Hit
GTATCAACGCAGAGTACATGGGGATCCGGAGGGATCACTTCAATTTGTGT15490.14626026017141439No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCTGTCTCTTATACAC14770.13946184911115497No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG14360.13559053170184057No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG14290.13492957507098202No Hit
GCATGAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC13570.12813116401072258No Hit
GTTCTTCTCCAGGAACTCGCGCACTGACTTGGCACCCCGGCCTATGGCAT13360.12614829411814693No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC13250.12510964798394064No Hit
GGACACAATCTGGCTAATAAGGCGGTTAAGGTTAGTGTAGGTTGGGCGCT13240.1250152256081037No Hit
TGCTTCCGGATAACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA13090.12359888997054967No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA12820.12104948582295239No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12710.1200108396887461No Hit
CGTCAGATGTGTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCAC12610.11906661593037672No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCCTGTCTCT12570.11868892642702897No Hit
GCGTCAGATGTGTATAAGAGACAGCATTGGCTTCCAGGCCCTGCACCAGT12560.11859450405119203No Hit
GTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTC12360.1167060565344533No Hit
CTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATC11930.11264589437346505No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG11660.11009649022586776No Hit
GTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCG11180.10556421618569482No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACCTGTCTCT11130.10509210430651014No Hit
TACCACCTCTTCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGG10830.10225943303140204No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)