FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004047514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences828978
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT11770214.19844676215774No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA484285.8418920646868795No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA155321.8736323521251468No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG121761.4687965181223146No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG110941.3382743570999471No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC103661.2504553799980218No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA77530.9352479800428961No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC62800.7575593079671596No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC56810.6853016605989544No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG53560.6460967601070233No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA52870.6377732581564288No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA49840.6012222278516438No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT49800.6007397059994355No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA47400.5717883948669326No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC45740.5517637380002847No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC42110.507974879912374No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT40800.4921722892525495No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC40290.4860201356368926No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38820.46828745756823464No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38190.4606877383959526No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA36890.44500577819918025No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT32600.39325530954983123No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC32540.39253152677151865No Hit
CGTCAGATGTGTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCAC29690.35815184480167145No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT29620.3573074315603068No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC28700.346209428959514No Hit
AACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATT25890.31231226884187513No Hit
CTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATC25850.3118297469896668No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACCTGTCTCT25770.31086470328525No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT21410.2582698213945364No Hit
GCGCGGGGACATTCAGTGCGCTGGGGAGGCCTCGGTGATGGCCCTCTCCA20360.2456036227740664No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC19920.24029588239977417No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCTGTCTCTTATACAC19650.2370388598973676No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC19590.23631507711905503No Hit
GTATCAACGCAGAGTACATGGGGATCCGGAGGGATCACTTCAATTTGTGT19520.23547066387769036No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCCTGTCTCT19110.23052481489255444No Hit
GTGTAGAAAATGTTACAGGTCAGTTTTCCTATCTGACCAGTGCACATATT17770.21436033284357364No Hit
GATGAAAGTGGTAGGGTTGCTGATAAGTGCAACATTTTTAACCAGCTGGA16820.20290043885362458No Hit
GTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTC16280.19638639384881143No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC15600.18818352236126895No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACCTGTCTCTTATACACATCTCCG15330.18492649985886236No Hit
GTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCG15250.1839614561544456No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCTGTCTCTTAT15010.1810663250411953No Hit
CAACGCAGAGTACATGGGATGACCCACCAATCACATGCCTATCATATAGT14850.17913623763236178No Hit
CCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGA14420.17394912772112167No Hit
GCATGAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT14080.16984769197735042No Hit
GTGTTAGGTGTTCGAACTGCTCCATATTGTTCATTATACTGCTCAGTAAA14030.16924453966208994No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14010.16900327873598575Clontech SMART CDS Primer II A (100% over 21bp)
GCGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTAT13710.1653843648444229No Hit
TACCACCTCTTCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAC13320.16067977678539117No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA13100.15802590659824506No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCT12950.15621644965246362No Hit
ACCTCAATCTGTCCAGGTGTGTCAATCAACACATATTTGGACATGTTCTG12930.15597518872635946No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG12590.1518737529825882No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG12590.1518737529825882No Hit
TGCTTCCGGATAACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA12360.14909925233239002No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG12130.1463247516821918No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCCTGTCTCTTATACACAT12050.14535970797777503No Hit
ATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATTGAAGT12040.14523907751472295No Hit
GGTATCAACGCAGAGTACATGGGGATCCGGAGGGATCACTTCAATTTGTG12010.14487718612556666No Hit
GAGTACATGGGAGCTCCGACAACAGGAATTTTCTCCGAGAGCGGGCCGGG11680.1408963808448475No Hit
GTACATGGGGATCCGGAGGGATCACTTCAATTTGTGTTCTGGTGCTATCC11400.13751872787938882No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG11320.13655368417497207No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG10710.12919522592879426No Hit
ACACTACCTTGAACTCGGCATCAGAGGGAGACCTGTCTCTTATACACATC10650.12847144315048167No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCTG9970.1202685716629392No Hit
GGGAGAGCACCATGAGGACCTGCACCGCCTTCAGCCAGCCATTCTCGCTG9640.11628776638222005No Hit
AGATCAAGCAGAGGCTGAAGCTGAAGGACGGCGGCCACTACGACGCTGAG9620.11604650545611583No Hit
GCATTTTGGGAGGCCGAGGCGGATGGACTGCTTGAGGTTAGGAGTTCAAG9350.11278948295370926No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCTG9230.11134191739708413No Hit
GTGTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCACCTGTCTCT9130.11013561276656317No Hit
GGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACA9110.10989435184045897No Hit
GATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACAT9030.10892930813604222No Hit
CGTGGTTTTTCTGCCTCCCGAACCAGAGCTGTCTCTTATACACATCTCCG8960.10808489489467754No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTCTG8900.10736111211636498No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG8890.10724048165331287No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGCTGTCTCTTATAC8870.10699922072720869No Hit
GTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTA8820.10639606841194821No Hit
CGATCGAACTGGCGGGCGGCGTGGCGCGCCAGAACGACTTTGGAGGCCTG8750.10555165517058354No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA8730.10531039424447936No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG8690.10482787239227097No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8680.10470724192921886No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAGG8580.10350093729869791Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)