FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004047515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192612
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA157588.181214046892197No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA104785.439951820239653No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG69823.6249039519863766No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA49862.588623761759392No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC44352.3025564346977343No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA29551.5341723257117936No Hit
CGTCAGATGTGTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCAC26501.375822897846448No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA25631.3306543725209228No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC22871.1873611197640852No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACCTGTCTCT22331.1593254833551387No Hit
CTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATC20591.068988432704089No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA16040.8327622370361141No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT15100.7839594625464664No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC12940.6718169169106806No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC12010.6235333208730505No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT11680.6064004319564721No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCTG11660.605362075052437No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC11380.5908250783959462No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTCTG11030.5726538325753328No Hit
GTGTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCACCTGTCTCT10080.5233318796336677No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA9980.5181400951134925No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC9690.5030839200049841No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC9580.4973729570327913No Hit
AACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATT9150.47504828359603757No Hit
GTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTA8180.42468797375033746No Hit
CCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGA7630.39613315888937345No Hit
CGTCAGATGTGTATAAGAGACAGCGTGGTTTTTCTGCCTCAGCCTCTGGA7630.39613315888937345No Hit
CGTCAGATGTGTATAAGAGACAGTGCTTCCGGATGACTGACGACTCTCAC7490.38886466056112806No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCTG7270.37744273461674244No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCCTGTCTCT7000.36342491641226926No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCTGTCTCTTATACAC6500.33746599381139286No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6440.3343509230992877No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6370.330716673935165No Hit
GTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTC6260.32500571096297215No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACCTGTCTCTTA6200.321890640250867No Hit
GATCACTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCG6010.312026249662534No Hit
ACTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAAT5950.30891117895042886No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACCTGTCTCT5610.29125911158183293No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAA5190.26945361659709677No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACCTGTCT5170.2684152596930617No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATCTGTCTCT5170.2684152596930617No Hit
GTGTATAAGAGACAGGCTCCAGAGGCTGTCTCTTATACACATCTCCGAGC5160.26789608124104414No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCCTGTC5020.2606275829127988No Hit
GTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCG4920.2554357983926235No Hit
GATGTGTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCACCTGTC4870.2528399061325359No Hit
TCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCAC4820.2502440138724482No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGCTGTCTC4700.2440138724482379No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCTGTCTCTTAT4590.23830290947604513No Hit
GCGTCAGATGTGTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCA4540.23570701721595747No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA4460.23155358959981726No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTCTGTCTCT4380.22740016198367705No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATCTGTCTC4370.2268809835316595No Hit
GGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACA4200.2180549498473615No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCT4200.2180549498473615No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACCTGTCTCTTATACACATCTCCG4170.21649741449130894No Hit
GCGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTAT4150.21545905758727388No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCCTGTCTCTTATACACAT4060.21078645151911615No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG3960.20559466699894088Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACC3950.20507548854692337No Hit
GTATAAGAGACAGCGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCT3910.20299877473885325No Hit
CGTGGTTTTTCTGCCTCCCGAACCAGAGCTGTCTCTTATACACATCTCCG3860.2004028824787656No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACA3840.19936452557473053No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGCT3800.19728781176666044No Hit
GGAGGGATCACTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGG3800.19728781176666044No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGC3560.18482752891823978No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG3390.17600149523394182No Hit
ATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATTGAAGT3370.17496313832990676No Hit
GATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACAT3340.17340560297385418No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT3160.16406039083753868Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTG3130.16250285548148607No Hit
CGTGGTTTTTCTGCCACAGCCTCTGGAGCCTGTCTCTTATACACATCTCC3130.16250285548148607No Hit
CAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGT3110.16146449857745104No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCCT3100.1609453201254335No Hit
GTATAAGAGACAGTGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTA2990.15523435715324072No Hit
GCAATCGCAGGCACTGACGACTCTCACCCTGTCTCTTATACACATCTCCG2910.15108092953710048No Hit
GCAGCGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCT2890.15004257263306545No Hit
AATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTT2870.1490042157290304No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTCT2810.14588914501692524No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACCTGTCTCT2760.14329325275683757No Hit
CGTGGTTTTTCTGCCTCAGCCTCCCGAACCAGAGCTGTCTCTTATACACA2730.141735717400785No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGCTGTCTCTTATAC2650.1375822897846448No Hit
GGGATCACTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTT2580.13394804062052207No Hit
GGATCACTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTC2480.12875625610034683No Hit
GTGTATAAGAGACAGCGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCT2470.12823707764832928No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT2420.12564118538824165No Hit
ATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTT2380.12356447158017153No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACCTGTCTCTTA2380.12356447158017153No Hit
ACACTACCTTGAACTCGGCATCAGAGGGAGACCTGTCTCTTATACACATC2370.12304529312815402No Hit
GTACATGGGGATCCGGAGGGATCACTTCAATTTGTGTTCTGGTGCTATCC2360.12252611467613649No Hit
GCAATCGCAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGC2340.12148775777210143No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCTGTCTCT2320.12044940086806638No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCTG2270.11785350860797873No Hit
GCAGCGTCAGATGTGTATAAGAGACAGGCAATCGCAGGCACTGACGACTC2270.11785350860797873No Hit
ATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCA2260.1173343301559612No Hit
GTATCAACGCAGAGTACATGGGGATCCGGAGGGATCACTTCAATTTGTGT2240.11629597325192614No Hit
TTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATTGAAGTG2240.11629597325192614No Hit
GCATGAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACA2220.1152576163478911No Hit
TGCTTCCGGATGACTGACCTCTCAAAGTGCTGGGACTGTCTCTTATACAC2200.11421925944385604No Hit
GTGTATAAGAGACAGTGCTTCCGGATGACTGACGACTCTCACCTGTCTCT2140.11110418873175089No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2010.10435486885552303No Hit
CGTCAGATGTGTATAAGAGACAGAGATGGGCACTGACGACTCTCACCTGT2000.1038356904035055No Hit
CGTCAGATGTGTATAAGAGACAGCGTGGTTTTTCTGCCTCTGGAGCCTGT2000.1038356904035055No Hit
CAGACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCAC1990.10331651195148797No Hit
CTTGAAGTTATGCGGCCGCCATTTGTCCGCATTGATCCAAGCGCTTGACT1950.10123979814341784No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCCTGTCTCT1930.1002014412393828No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)