FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004047516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1058533
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT504794.768769608505356No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA109541.034828389856528No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC99950.9442313088018985No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80970.7649265540139042No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA67620.6388086153195035No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG65200.6159467867322039No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62330.5888337916720593No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT62040.5860941510562259No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC60810.5744742960304497No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT53710.5074003361255625No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA47410.4478840055057329No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG39430.3724966533872822No Hit
GTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGC38520.36389985007552905No Hit
AGCTAAGACTACAGGTGCATGCCACCAGGCCCAGCTAATTTTTGTAGAGA38110.360026565066937No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC37830.3573813948171668No Hit
AGACCATCCTGGCTAACACGGTGAAAACCTGTCTCTACTAAAAATACGAA35070.33130757378371767No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC34650.32733981840906234No Hit
GTACATGGGAGGCTGGCCTTGAACTCCTCCTAACCTTAAGCAATCCACCT34620.3270564073108727No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC33310.31468078935659066No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA30520.2883235572249519No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC29610.27972675391319873No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG28940.2733972393869629No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGCTACCCTTGGGATAACAG28120.26565066936977877No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT27090.25592022166526696No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC25990.24552848139831254No Hit
GTTGGAAGCCGCTGATGCTGGCTTGGACTGCTGCGTGGCAACATGCGGTG25950.2451505999340597No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG25710.2428833111485424No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCC25180.23787638174719164No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23470.22172194915038076Clontech SMART CDS Primer II A (100% over 21bp)
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG22850.21586478645446106No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC22770.2151090235259553No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG21310.2013163500807249No Hit
GCAGGCAGCCAACCGCTCTACACAGCTCAGCTGTTCCCAGTCTGCTCCTG21160.19989929458977662No Hit
GAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGCATGC20070.18960202468888546No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA19570.1848785063857244No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA19460.18383933235902897No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA19190.18128863247532198No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA18510.17486464758302292No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG18360.1734475920920746No Hit
CTGCTTATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCA16880.1594659779147178No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA14990.14161107872876896No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG14760.1394382603093149No Hit
GCATAATATTTCGCCCACTAAGCCAATCACTTTATTGACTCCTAGCCGCA14600.13792673445230333No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14420.13622626786316533No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG14040.13263639395276292No Hit
GAATTGGGAAAGCTCATGGAGCTTCATGGTGAAGGCAGTAGTTCTGGAAA13870.13103039772968816No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA13620.12866863857810765No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC13570.12819628674779152No Hit
TGCTTCCGGATGACTGACCTCTCAAAGTGCTGGGACTGTCTCTTATACAC13540.12791287564960185No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC13120.12394512027494656No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA12720.1201663056324177No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG12680.11978842416816482No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG12210.1153483169631934No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC12000.11336443927586574No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG11230.1060902210889977No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG10960.10353952120529072No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCACCACGTTGC10730.10136670278583662No Hit
ATCAAGCACACCAACATACCTATAATGGAATCTCAAAAGAAAGAGAGAAA10690.10098882132158374No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)