FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004047517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2465920
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG1136704.609638593303919No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA252221.0228231248377886No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC189130.7669754087723851No Hit
GTACATGGGGAGTTCATCATGCTGATGGCGAGGCTAACCTGGGCCTCCCA179470.7278013885284194No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA169410.687005255644952No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC160060.6490883726966No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151160.6129963664676875No Hit
CCACAAAAACTTAGCCAGGCATGATTGGCATGTGCCTGTAGTCTCAGCTA142070.5761338567350117No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG137450.5573984557487672No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT124730.5058152738126136No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116600.47284583441474176No Hit
GTAAGGAAGGGATCCAGTTTCAATTTTCTACATATGGCTAGCCAATTCTG114470.4642080846093953No Hit
GTGAACAAAGGTCTCTGGTTTTCCTAGGCAGAGGACCCTGCGGCCTTCCG101700.4124221385933039No Hit
ACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGC98140.3979853361017389No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA97650.3959982481183493No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT97470.39526829743057357No Hit
TTATTCCTCTTGGCTGGAGAAGAGAACTAGTGGGTGGTTGTGTACAGGAC96850.39275402283934596No Hit
CCCTGGGGCGGAGCCCGGCCCTCCCTGCATTCCTGCAGAGCCTCGGTCCA75510.30621431352193096No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA75100.30455164806644175No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT74380.3016318453153387No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC68610.27823287049052686No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC68090.2761241240591747No Hit
CACATAACATGAGCACACACACAAAATACACACAACCACCAAAATACACA67930.275475279003374No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA67260.2727582403322087No Hit
GCAGAGAGGTTGGTTGATCCCTCAAGATGCGCAATGATTTCTTTCTTCCC64740.26253893070334805No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG64060.2597813392161952No Hit
GGAGAAGAGGAAAGAGAAAGCCAAGATCCACTACCGGAAGAAGAAACAGC62680.25418505060991436No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT61030.2474938359719699No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG60820.24664222683623152No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC60700.246155593044381No Hit
GTTGTAGGTCGAAGCCATCGTCGCACCATAGGCGCCCGCGGTGCGGAACA55490.22502757591487152No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC52240.2118479107189203No Hit
GGACAGACCTGTCCAAACCCCTTATACATTTCAGCTGTCAGAGTGAGGTC51680.20957695302361795No Hit
AGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACA51310.2080764988320789No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC48380.19619452374772905No Hit
AAACAAGATGAGATTGGCATGGCTTTATTTGTTTTTTTTGTTTTGTTTTG45920.1862185310147937No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG44690.18123053464832597No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG42300.1715384116273034No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG40880.1657799117570724No Hit
TGTTTGGCCCTGCCAAGTCTCATGTTGCAATTTATTCTCCACTGTTGAAG39650.16079191539060472No Hit
GCTGGGATTACGGGCGCCCGCCACCACGCCCGGCTAATTTTTTGTATTTT39440.15994030625486633No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA36640.14858551777835455No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35130.14246204256423567Clontech SMART CDS Primer II A (100% over 21bp)
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC34900.1415293277965222No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG34210.1387311834933818No Hit
GGAAAGAAGGAAGGAAGGAAGGAGGGAGAGAAGAAAGGAAGAAGAGAAGA34020.1379606799896185No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC33240.1347975603425902No Hit
AAACCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTTGCAAAGCTGAAACT32930.1335404230469764No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG32470.13167499351154943No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA31920.12944458863223463No Hit
CTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTA31440.12749805346483262No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA31190.12648423306514403No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC30610.1241321697378666No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC29720.12052296911497534No Hit
GCAATAGTGCGTGCCTGTAGTCCCAGCTACCCGGGAGACTGAGGCGGGAG29150.11821145860368544No Hit
ACGCAGAGTACATGGGAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT28790.1167515572281339No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG28190.11431838826888138No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG28010.11358843758110564No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27890.11310180378925512No Hit
GAGAACCACCTGTGCTGTTGTGGAAGGCGTGCCGTTGAGGGGGAAAACGA26610.10791104334284972No Hit
CAAATACTGCTCCCCATTTTTAAGCATTAAGAACCGAAGTTCAGAGTGGT25640.10397742019205815No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA25020.10146314560083051No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)