FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004047518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2246849
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG806253.5883586302417294No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA207280.9225364054282241No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC202030.8991703492313011No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164520.7322254410510007No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC154890.6893654179697879No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA144750.6442355494294454No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT129500.576362719524098No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127840.5689745950884996No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT112610.5011907787305689No Hit
GGATCTAAGCGGTGAGTGAAGGAAGAACAAAAGAACCATTCTCAGAAGCA106960.476044451585309No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG105210.46825576618633474No Hit
CTTTGGAGCTTCTGAGGACTCAGCTGGAACCAACGGGCACAGTTGGCAAC102080.4543251460155978No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG94240.419431835428193No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC89910.4001604024124451No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC75710.3369607837464823No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA63560.28288505369074646No Hit
GCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTG59550.2650378374336682No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG58410.2599640652309078No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA57200.25457874561218846No Hit
CACCATAACAGATATAATAGTAGTGAAAAAGTGTGAGGTATTATGAGAAT56570.2517748188685577No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG55600.2474576618188405No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT55470.246879073760631No Hit
CTCTGGGGCTTCAAAGGGATCACGGTGCTTGTTTCCAACACTATACTCGC54960.24460922830150134No Hit
GCCTGGTGGTGCATGCCTGTAATCCCAGCTACTTGGGAGGCTGAGGCAGG54800.24389711992216656No Hit
TGGCAGAGATACCTTTATTTGGAATTGGTAGCAATAGGGGAAGATACGTG54380.24202783542641274No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC53800.2394464425513241No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC52790.23495125840677322No Hit
GTGGTATCATCGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCA48210.21456715604831478No Hit
TGGGGAAGCAGTCCAGACTAGGCTGGACTTTGAAAGAGGGCCCACAGGAA47160.2098939448089302No Hit
TCCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCT46520.20704551129159102No Hit
GTACATGGGACCTGAAAGACTGGGTGGTGGGCTGCACTACAGTAGACCAT45660.2032179287526665No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG44950.20005794781936836No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA44750.19916781234519987No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA43800.19493966884289954No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43210.1923137691941025Clontech SMART CDS Primer II A (100% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG42730.1901774440560981No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC41070.18278931962049963No Hit
ACTCGGGAGGCTGATGCAGGAGAATGACTTAAACCCGGCAGGCAGAGGTT39940.17776005419144766No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC35580.15835510085457455No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG34400.15310330155698046No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA33180.14767347516455268No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30050.13374285499381577No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG29080.12942569794409858No Hit
GGATGATCTCGATCTGACCTTGTGATCCGCCCGCCTCTGCCTCCCAAAGT27340.12168151931883273No Hit
GCCCAGCTCATTCCTCACGTCGGCCATTCCAGGCCCTGTTTTTCCCTGTC27130.12074687707095581No Hit
GACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTG27040.12034631610758No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCC26740.11901111289632725No Hit
GAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCG26060.11598465228415439No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA25910.11531705067852802No Hit
ATTTAAAGGAGCATCTCAGTTACGTGTGGCGGACAAAAAAAAGGAGCAGA25830.11496099648886063No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC25530.1136257932776079No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC25480.11340325940906576No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC25270.11246861716118885No Hit
TCACCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGT24320.1082404736588885No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA24100.10726132463730319No Hit
TTCCTTTTGTGGCTGAATATGTACTCCATTATGGCTACATCATATTGTTT23020.10245459307679332No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG22670.10089685599699848No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)