FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004047519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1187089
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA439323.700817714594272No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC137921.161833695704366No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA135181.1387520228053667No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99500.8381848370256991No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87320.7355809042118999No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA87160.7342330692980897No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC79310.6681049188392783No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT78130.6581646363499283No Hit
ACCAGGAGTGGAGCCTGCGGCTTAATTTGACTCAACACGGGAAACCTCAC77880.6560586442970998No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT76830.6472134776752206No Hit
GCTCATTGCAACCTCTGCCTCCCAGGTTCAAGTGATTCTCCTGCCTCAGC69450.5850445922757266No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA60470.5093973577381309No Hit
CATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGA58490.49271790067973No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG55940.4712367817408804No Hit
ACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAAC52100.4388887438094364No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG50980.4294538994127651No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC47120.3969373821170948No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC44910.3783204123700919No Hit
GGTTAATTCCATTCCATTCCATTCCATTCCATTAAAATCGAGTTGATTCC44690.3764671393636029No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC41420.3489207633126076No Hit
ATTGACTATATTTTTTAAGTAGTTGTATTAATCTCTATCTTTCAAAGAGA40310.3395701585980495No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC40180.33847504273057877No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC37840.31876295711610503No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT36010.30334709528940124No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG35260.29702911913091606No Hit
TATATACTGGTTGAGTTACGTTTACATTGCTCCATAGGACAATCCCATGA35220.2966921604024635No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32970.27773823192700803Clontech SMART CDS Primer II A (100% over 21bp)
CTTCCGAAAGGAGCTGGTGAATGCTCAGTGTGCGAAATTTATTGATGAAC31230.2630805272393224No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG28010.23595534959889275No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG27920.23519719245987453No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG27690.2332596797712724No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC27460.2313221670826703No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA27190.2290476956656156No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACG25460.2144742306600432No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACC25450.21438999097793005No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG25350.21354759415679866No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA25190.2121997592429885No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA22090.1860854577879165No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG20270.1707538356433258No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19940.16797392613359233No Hit
GAGTACATGGGAGAGAGTGGTAGTTGACAAGTGGGGGCAGGGATCAGATC19290.16249834679623854No Hit
ACGCAGAGTACATGGGAAGGTAGCCAATTGCCTCGTCATCTAATTAGTGA19010.16013963569707074No Hit
ATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCT18430.15525373413450888No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG18160.1529792627174542No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC17480.14725096433376098No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA16000.13478349138101692No Hit
GAGGTTGAGGCTACAGTGAGCTGAGATAGCACCACTACACTCCAGACTGG15440.13006606918268135No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG15180.1278758374477398No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA15150.1276231184014004No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC14400.12130514224291523No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG13990.11785131527627668No Hit
GGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCA13730.11566108354133516No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT13670.11515564544865634No Hit
GTATCAACGCAGAGTACATGGGGATCCGGAGGGATCACTTCAATTTGTGT12790.10774255342270041No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC12080.10176153599266778No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)