FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004047521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2707640
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA1536935.67627158706475No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA274981.0155707553441373No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC234940.8676928986128142No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG188220.6951441107385029No Hit
GGATGGAGGGAGGCTGCTACTCTACCACTTCCTGTGTGCCTCTACTGTGG169460.62585868136089No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA164790.6086111890797891No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163850.6051395311045782No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT158710.5861562098358719No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC151460.5593801243887666No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT137980.5095950717229765No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA130080.48041837171854457No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129510.47831321741442734No Hit
GCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAA113530.41929503183584227No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC94980.3507851856229041No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG87600.32352897726433355No Hit
GTGGTTATCACTAGGCTAAAACTGGACAAAGACCGCAAAAAGATCCTCGA87260.3222732711881934No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC86500.3194663987827038No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC79040.29191473017092373No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG73750.27237742092744976No Hit
GTCATCAAGTGGCCTGATGGATCCAGGGCATGGGAAGGTGGAATGAGGGA72910.26927508826875063No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT66100.24412403421429732No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC63170.23330280244050172No Hit
GTCCACGTGCAGCATCCGGAAGGCGCGGGGGTTGAGGCCGGCGTGGTGTG62470.2307175252249191No Hit
GCCAGTATTCTGGCAACCAAAGAAAAAACAGCCTCTGACTCGTTGACCCC59980.2215213248437754No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA59060.2181235319318669No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC58480.21598144509609846No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC57600.21273138231079464No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG54940.20290732889158086No Hit
AGGCTGGAGTGCAGTGGCGCGATCTCGGCTCACTGCAAGCTCCGCCTCCC54780.20231640838516196No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG54240.20032205167599829No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG48090.17760854471052281No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG43350.16010252470786368No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC42680.15762804508723463No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG42540.15711098964411813No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA42370.15648313660604807No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41630.15375012926386078Clontech SMART CDS Primer II A (100% over 21bp)
GGCCAAGGCGGGCAGATCACCTGAGGTCGAGAGTTTGAGACCAGCCTGAC38250.14126693356576206No Hit
CCCTCAAAGGGAAGCCCATCAGACTAACAGCGGATCTCTCGGCAGAAACC36430.1345452128052474No Hit
GACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATG36090.13328950672910728No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG35390.1307042295135247No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC34450.1272325715383138No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA33600.12409330634796355No Hit
TCCCCTGCTCGTCGATCATGCCGCAGAAGAAGAACCCCGACGTGCCCGAG33450.12353931837319584No Hit
GCTTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAG32500.12003072786633379No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31080.11478630837186626No Hit
AAGGTGAGGGGGCCCCTGGGAATTCTGTGATTGGTCTGGGAAAGATTAAA30740.11353060229572617No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG30720.1134567372324238No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA30210.11157317811821366No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC30080.11109305520674831No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG29990.1107606624218877No Hit
CATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGG29860.11028053951042235No Hit
CTTCCTCACCATTTTGCTTCACTTTTTTTTTTTAACTGCTTCCTTTGTGT29580.10924642862418933No Hit
CTGCAAGCTCTGCCTCCCGGGTTCACACCATTCTCCTGCCTCAGCCTCCC29040.10725207191502563No Hit
GTATGAAGCCTCGAGATCGATGCCCTGCGCCTTCTGCACCGCGAAATTGA28130.1038912115347683No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC27910.10307869583844234No Hit
TCCCTATGTTGCTTAGGCTGGTCTCGAACTCCTGGCCTCAGGTGATCCCC27600.10193378735725578No Hit
GGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCG27440.10134286685083689No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA27350.10101047406597627No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)