FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004047522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences427788
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCGCCGCGAGGCGAGCCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCG46571.088623336792991No Hit
CTATAATCCCAGTACTTTGGGAGGCCGAGGTCGACAGATAGCTTGAGCCC35970.8408370501276333No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAAT35880.8387332042974557No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26140.6110503333426838No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25580.5979597370660233No Hit
AACGCAGAGTACATGGGGTACAGTGAAACTGCGAATGGCTCATTAAATCA21910.5121695793243383No Hit
AGTCAGAGAGAGAGAGAGCGAGTTAAAGAGAGAAATGGAGTTAGTTCTCA20740.48481958353202986No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA19380.45302813543156895No Hit
GAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCG18790.43923625721151593No Hit
CAAATGGGAGTCACTGGAAGGTTCTGATGAGAGGACCGAAATGAACGGAC15380.35952387631256605No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA15030.35134225363965327No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC14800.3459657587403106No Hit
GTCGACGACACCGCCACATAGGTCGGCACGGCCTGCACGTTGAAGGCGCG14540.33988798189757546No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG13950.3260961036775225No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC12270.2868243148475413No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA11400.26648713848915817No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA11400.26648713848915817No Hit
CATTTAAACTGAGATCATTGCTAATGGTTAATGAGTCAAGGCGTACTCAG10510.24568244083518007No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC9990.23352688714970968No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9960.23282560520631715Clontech SMART CDS Primer II A (100% over 21bp)
GTGACATCCAAACAATAAATATGCAATAAATAGCGCTCCTGGGCTGGGCC9830.22978671678494955No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT9690.22651406771578447No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT9410.21996876957745426No Hit
CTTGAACTCCTAGCCTCAAGAGATCCTCTCGCCTTGGCCTCCCAAAGTGC9140.21365723208692156No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT8920.2085144978353764No Hit
TTGGGAGGCCGAGGCGGGCGGATCACCTGAGGTCAGGAGTTCGAGACTAG8770.20500808811841378No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG8350.1951901409109185No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA8320.19448885896752596No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACC8080.1888786034203858No Hit
CCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTC8040.18794356082919578No Hit
ACACTGGTCTGGAACTCCCGTCTGGCCACCTGGAGTGGGAAGTTGCTGTA7460.17438544325694033No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG7350.17181407613116778No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA6900.16129484698027993No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG6640.15521707013754477No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG6420.1500743358859996No Hit
CTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGA6120.14306151645207438No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT6110.1428277558042769No Hit
GGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAG5780.13511365442695913No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC5710.1334773298923766No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC5670.13254228730118656No Hit
CCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATG5510.12880211693642646No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGT5480.12810083499303393No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCC5310.12412690398047631No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT5250.12272434009369126No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC5210.12178929750250125No Hit
CTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGA5200.12155553685470374No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC5090.11898416972893114No Hit
GTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTT4880.1140751961251835No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGC4880.1140751961251835No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG4870.113841435477386No Hit
GTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTGG4670.10916622252143585No Hit
CTTTATAAGAAAGGACATTGGCCGGGCGTGGTGGCTCTCGCCTGTAATCC4640.10846494057804333No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT4630.10823117993024584No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT4620.10799741928244833No Hit
GAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTT4580.10706237669125829No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGA4530.10589357345227074No Hit
CCCTGAACCCCCAGCCCTGAAATCCCCAGTAAGGAAACAGGACCCGCGGC4480.10472477021328322No Hit
GGATGGTCTCGATCTCCTGACCTTGTGATCCACCCACCTCGGCCTCCCAA4410.10308844567870065No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4410.10308844567870065No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA4370.10215340308751064No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)