FastQCFastQC Report
Sat 10 Sep 2022
EGAF00004047524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2903169
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT1231294.241192986009426No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA287340.9897460326973732No Hit
GATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGG211960.7300987300429289No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC177480.6113319617287177No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG159900.5507774435453121No Hit
GGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAGT146730.5054132225853886No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140790.48495282224355524No Hit
ATTTTTTTCTGTATTTTTAGTAGAGACAGGGTTTCACCATGTTAGCCAGG140210.48295500537516073No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA137710.47434372577001205No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT133820.4609445747044006No Hit
ACAAAGCGGAAGGCAATATCCAAGCGGCATATGGCAAGCTCAAGGCGATC133370.45939454437547383No Hit
ATCTTGCGCAAGTTGGTCACGTGGTCACCCAATTCTTTGATGGCTTTCAC127610.4395541561652112No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC123770.42632723069170275No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109590.37748405277129926No Hit
GTCCCTTGCCATCCTAAAAGCCACCCCACTTCTCTCTAAGGAGAATGGCC102030.3514435432453295No Hit
GTCATAACCAGCCTGGCAGTATGATGGCCTAGATGCAGAGAAAACAGCTC101100.3482401472322142No Hit
CTTTTGAATAGGTCATGGTTTTCCTGCGTGACATCATGAGCCACCAAATA99720.34348672089017207No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA99510.3427633734033396No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT95330.32836531390353096No Hit
TGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTGC92580.3188929063378673No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC89380.307870468443277No Hit
TACTAACAGGCCGAGTGGAGAAACCCACAAGCCTGTCTGTCCAAATTCTT78590.27070418566745513No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG77320.26632965562803956No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA76050.261955125588624No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC74310.2559616749834405No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC69860.24063359728627576No Hit
GAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGC58600.20184839394468596No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG57990.19974724172102967No Hit
TTTTTTTGGGACAGAGTCTCATTCTGTCACCCAGGTTGGAGTAGAGTGGC57020.19640606523423196No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA56640.19509715073424938No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC56010.1929271082737519No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC54990.1894137061948512No Hit
GAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTT51690.17804681711605488No Hit
CTCCCAGGGTGTGCTTGTCAAAGAGATATTCCGCCAAGCCAGATTCGGGC50400.17360339683979817No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT48990.16874663514249427No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC47090.16220206264258125No Hit
GTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAG46850.16137537980048697No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG43630.15028405166905542No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGT42940.1479073384980344No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTGCCTACCC42790.14739066172172546No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG38060.1310981207087841No Hit
AACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGC37270.1283769563535571No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG36460.1255869017614889No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGG36040.12414020678782392No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG35890.12362353001151499No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC35130.1210057010115498No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA33700.11608004907740473No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33650.11590782348530175Clontech SMART CDS Primer II A (100% over 21bp)
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC32090.11053438501168895No Hit
GCAATCGCAGGCACTCGGCAGGCTGAGGCAGGAGAATCAGGCAGGGAGGT31240.10760654994593839No Hit
GCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAG30890.10640097080121756No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG30750.10591873914332924No Hit
GATTGTTCCTATGTTTTTTCAACGTGTCTTCTGCTTTTCCACTCGAGGAG30470.10495427582755258No Hit
GCCAATGATTTAATCAAGCATGCCTATGTAACAGGATCTCCATAAATCCC30270.10426537345914068No Hit
CAACGCAGAGTACATGGGCCGGTCCCTCCCAGTGGTGCGTCCCCGTACGA30180.10395536739335531No Hit
CGTGGTTTTTCTGCCTCAGCCTCCAAGCAGCTGGGATTACAGACATGAAC29830.10274978824863451No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG29620.10202644076180202No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG29390.10123420303812833No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)