FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004047525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1770529
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC307131.7346792964136708No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA140270.7922490961740812No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC130540.7372937692633106No Hit
GTACCAGTCCAATACCATCCTGCGTCACCTGGGCCGCACCCTTGGGCTCT121170.6843717329679435No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115240.6508789181086556No Hit
ACCAGAAACCCCTGAGGGCCGGTTACTGACCTCAAAAATCATGACTTTGG114390.6460780930445081No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA101990.5760425274028271No Hit
GATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGG98130.5542411335821102No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92980.5251537817228636No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT88480.49973764903031803No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC86150.48657774032506673No Hit
CCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGG85010.48013898670962185No Hit
CTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGA80870.4567561446324799No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA78590.44387863740159017No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT72600.4100469407730684No Hit
GGGTAATTTGCCCGCCTGCTGCTACATCCTTGGATGTGGTAGCCGTTTCT66740.3769494879778868No Hit
GTCGTCCGCCGCGAAACCGGGCGGATCAAGCACGTGGCGCAGATGCCTGA61370.34661956963144913No Hit
GTCCGTGTCCGGGCCGGGTGAGGTTTCCCGTGTTGGGTCAAATTAAGCCG57540.32498761669534926No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC57280.32351912902866886No Hit
GATGGAGTTTCGCCCTGTTGGCCAGGCTGGTTTCGAACTCCTGACCTCGT53650.3030167819900154No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG52060.294036415105316No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC51650.29172072301555074No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT49800.2812718684641709No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG49620.28025522315646906No Hit
AACAAGAACGGCCCTTTATGGCCAGCCTTGAACATGTCTTCAGTGGTGTC49050.2770358463487466No Hit
GTCCTTCCTCTTTACTTCTTCAGAAAGCATGTGCCGATAGGGAGTGGAAA48460.27370350895127954No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG47070.2658527479640266No Hit
GTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGC46110.26043063965628355No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA43210.24405135414330972No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA42970.24269582706637396No Hit
AGGGCTCTCAGTCACGGAGAAGGCACTTAAATGGGAAGGCCACCTGATGC42440.2397023714381408No Hit
TCATAGCAGTGACAGCCAGACCCAGCAAGATGGCTGCGACCGTGAAACCC41060.23190809074576016No Hit
GAGTACATGGGAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGA39550.22337956622003932No Hit
ATCTACAAGCACTGTGGCTATTGGCAGCTGTCACTGATACCAAATAGAAT37040.20920301220708612No Hit
CACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGT34380.19417925377104808No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC34230.19333204934796325No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC33830.19107283755307028No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA33540.18943490900177293No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG31300.17678332295037244No Hit
GTCCTGGTCATTCAGAAGGCTCCTATGATCCCACCAGTCTGCAGTCATTA30730.17356394614265003No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29770.16814183783490697Clontech SMART CDS Primer II A (100% over 21bp)
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC27680.15633745620659134No Hit
CGCCTGGGGAAGGGAGGGGGCCCTGCGGTATGAGGAAACACCTGCGCGCG26860.15170607202706082No Hit
ACTTGGGAGGCTGAGGCAGGAGAATCACTGGAACCTGGGAGGTAGAGGCT26820.15148015084757155No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG26080.1473006090270196No Hit
ATGTTGCCCAAGCTGGTCTGGAACTCCTGGCCTCAAGCGATCCGATCACC26000.14684876666804103No Hit
TTTCCCGGCTCACCTGGTTCAGGAAGCAGAGGAAAGCTGTGGTCAGCACC25570.1444201139885311No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG25130.14193498101414886No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGC24750.1397887298090006No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA24480.13826376184744785No Hit
TCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGG24070.1359480697576826No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACT23890.13493142444998077No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC23840.13464902297561915No Hit
ACTAATCGTGTATTGAAAGCAGTAAGAGGTTCCACGACACCAAATAGACC23300.13159908705251366No Hit
AACTCCATCTCTATAAAACATAAAAAAAATTAGCTTGGCATAGTAGCACG23280.13148612646276903No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT23150.13075188262942883No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22790.1287185920140252No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG22670.1280408284755573No Hit
TTTGTGCCCTGTACGCCATGATGACCCTAAGGAAAAAGCCCATGTACTCT22510.12713714375760013No Hit
GGAGTGCAGTGACATGATCTTGGTTCACTGCAACCTCCACCTCCTGAGTG20370.1150503606549229No Hit
CGGAAAATATGACTCAACCCACACTGTCTGCCCCTGATTGGCTGCTACAG20160.11386427446260412No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC20120.11363835328311482No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG20110.11358187298824249No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA19970.11279114886002997No Hit
ACTCAGGAGGCTGAGGTAGGAGGCTCACTTGAGCCCAGGAGTTCGAGTCC19860.11216986561643441No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG19630.11087081883437097No Hit
CTGATGTTCGCCACGAACCGCGCCTGGGGCAATTCGCCCGGGAGGGTGTG19390.10951529175743521No Hit
TCCATGAGGGATTCCTGGCCGGGTGTGGTGGCTCACACCTGTAATCCCAG19270.10883752821896732No Hit
GGCTCTCGCTGCCACTTCATCCACAACCCTAGCGAAGACCTGGCGGCCCC19220.1085551267446057No Hit
GCATCAAAAAGCTTTATTTCCATTTGGTCCAAGGCTTGTTAGGATAGTTA19070.10770792232152086No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC18340.10358486079584124No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)