FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004047526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1934761
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC829734.288540031559453No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA204931.0592005937684292No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC188460.9740738003298598No Hit
ACCTGCTCCAGCAGCTGGTGCTGCACCAGCAGGAGGTCCTGCCCCCTCCA160110.8275440739192076No Hit
GGAGAGGAGCGAGCGACCAAAGGAACCATAACTGATTTAATGAGCCATTC139570.7213810904809431No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA138420.7154372038716927No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT136590.7059786712674072No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125150.6468499209979941No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC121830.6296901787869406No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG116770.6035370777062387No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT103990.5374824073877859No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102490.5297295118105028No Hit
GTGCATGTCTCTGTGAGATTTTTCAGAGGCTGATGCTGGAAGGTGGCGGG101410.5241474269948588No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA88230.45602531785579714No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC73750.3811840325497568No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC72830.37642892326235644No Hit
GGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAGT72560.37503340205844543No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG65900.3406105456953081No Hit
GGAGAAAAAAGAGGGGTCGCAGGGGGCGAAGGCTGGGCGGCAGGGCCGAG63700.32923963218195945No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC57870.29910671137158545No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG51180.2645287970969024No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC51070.26396025142123497No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC50370.2603422334851695No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT49320.25491520658107125No Hit
TCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTATTCCTAGCT44160.2282452457952171No Hit
AGGCTGAGGCAGGAGAATTGCTTGAACCCAGGAGACGGAGGTTGCAGTGA42380.2190451430435077No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG42340.2188383991614468No Hit
CTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGA42080.21749456392805105No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC39660.20498655906336755No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG38700.2000247058939063No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA36780.1901009995549838No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG34170.17661096125051104No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA33340.17232102569774768No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30840.15939953306894236Clontech SMART CDS Primer II A (100% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG28170.14559937894137828No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA27470.1419813610053128No Hit
CTTTCGCACTTGGATCTTCAGTCGACAGTAGAGGAGGGTGAGGACAATTC26570.13732962365894288No Hit
GTTTCACCATGTTGGCCAGGCTGGTCTTGAACTCCTGACCCTGTGATCCG25710.13288463019463387No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA25650.13257451437154252No Hit
GTGTTAGCCAGGATGGTCTTGATCTCTTGACCTCGTGATACGCCTGCCTC25600.13231608451896643No Hit
CTCCCTATGTTAGTTGTAGCCCACAAGGGTCTAGGGTCTTTCCCATGACT24530.12678568567383774No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24390.12606208208662464No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA24270.12544185044044198No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG24060.12435644505962234No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCTGTCT22650.11706872321697616No Hit
GGCTGGTCTGGAACTCATGATCTCAGGTGATCCACCCGCCTTGGCCTCCC22390.11572488798358041No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC22340.1154664581310043No Hit
GCATATCAATATTAAAAAGCAAGCAAGCAGAATTTGGAATTCATCCAATC22150.1144844246912151No Hit
GAATCTTGCCCTTAACTTGTTTGTTTACAACAATGCCAACAGCATGCTGG20810.10755850464217545No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG20070.10373374282404907No Hit
TAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTGCC20050.10363037088301863No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA19900.1028550813252903No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)