FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004047528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences748731
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT94851.2668101093717237No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC54960.7340419990624135No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA40020.5345043814133514No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC37900.5061898064859075No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA37380.499244722069742No Hit
ATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGA34320.45837557146692204No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT32060.4281911661197413No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT30970.41363320070893284No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30460.40682167560846283No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA30240.4038833706631621No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC24970.3334976112916388No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24820.33149422155620645No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA22210.2966352401596835No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG20710.27660134280536No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC20410.2725945633344953No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC20080.26818710591654416No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT18020.24067388688327318No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG17360.23185897204737083No Hit
GGATGCAGTGAGCCGAGATCACACCACTGCACTCCAGCTTGGGCGACAGA17210.22985558231193845No Hit
GGGCAGGAGAATCGTTTGAACCCGGGAAGCAGATGTTACAGTGAGCCGAG16380.21877015910921277No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG16120.21529761690113006No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA15110.20180812601588555No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC14420.19259253323289674No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC14380.19205829597011476No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA14230.1900549062346824No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG13290.17750033055930634No Hit
GAAGAAGGCCGGGCATGGTGGCTCACGCCTGTAATCCCAGCACTTTGAGA12730.1700210088803589No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG12670.16921965298618596No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC12050.1609389754130656No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC11870.15853490773054676No Hit
GAGCTGGACCCCGAGAGCCGGGAGTACTGGCGGCGGCTGCGGAAACAGAA11840.15813422978346028No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11480.15332609441842263Clontech SMART CDS Primer II A (100% over 21bp)
ACTTGAACCTGGGAGGCAGAGGTTGCAGTGAGCTGAGATCATGCCACTGT11180.14931931494755793No Hit
GCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT10540.14077151874304655No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA10320.1378332137977458No Hit
TGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTTGCAA10060.13436067158966303No Hit
GGCGTAGGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCC9790.1307545700658848No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG9790.1307545700658848No Hit
GGCTGGAGTGCAGTGGCGCGATCTCGGCTCACTGCAAGCTCCGCCTCCCG9750.13022033280310286No Hit
GAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTT9700.1295525362246254No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA9670.12915185827753894No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC9380.12527863812236972No Hit
ATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCCCGTCC9210.12300812975554638No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT9210.12300812975554638No Hit
GTTTCACCATGTTGGTCAGGCTGGTCTCGATCTCCTGATCCCAGGTAATC9140.12207321454567796No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC9120.12180609591428697No Hit
GGGATATACTTTGTAGGTAGTATTCTAGCCCCACCCCATCCCCAAACTCA9120.12180609591428697No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC8370.11178914723712521No Hit
TATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC8360.11165558792142972No Hit
GTCTGTAAGTTACAAAGTCAGGAAAAGTCTTGGCTGGACCCCTTTCCTGC8280.1105871133958658No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGA8220.10978575750169287No Hit
ACCCGCCCGACACACGTCGCGCAGGTCATGCCATCGATGGCCAGCGACAG8120.10845016434473796No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG8080.107915927081956No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA8070.10778236776626052No Hit
ATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACG7890.10537830008374169No Hit
AGCCAGGATGGTCTCGATCTCCTGACCTTGTGATCCACCCACCTCGGCCT7750.10350846966400483No Hit
CCTTCACTGTGTGTACACAGCAAGAGCATGTGTGTGCCACTTCCCCTACC7720.10310779171691835No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGT7520.10043660540300856No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG7490.10003592745592207No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)