FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004047529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1202239
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG113720.9459017716111356No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC92660.7707286155248665No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86830.7222357617744891No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA84200.7003599117979037No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74260.6176808438255622No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT64790.5389111482824962No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC64540.5368316948626687No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT57720.48010420556977446No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG52740.43868149344681046No Hit
GCGTAGGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCC49580.4123972022201908No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC48250.4013345100267085No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC40400.3360396726441248No Hit
GTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGA36640.30476469320991917No Hit
CACTGGGTCTTTGTCTTTCTTGGCCGACTTTCCAGCGTCCTTCTTCTTCT34380.2859664342946785No Hit
GGCCGTCCCTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAA34170.2842196934220234No Hit
CTTTAGTAGGGTTTTGCCATGTTGGCCAGGCTGGTCTTTAACTCCTACCT32750.2724083979974032No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT32220.2679999567473689No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC29460.24504279099247323No Hit
GTTGTGGATGTTAATAAGCATTTGAGCTGGGTGCAGTGCCTCATGCCTGT29300.24371194080378358No Hit
TCTCTATCTTTCAAAGAGAAAAAAACTTATCTGCGGTTTCCTCAAGCTCC29190.2427969812990595No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA29090.24196519993112847No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG28480.23689133358674938No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27250.22666042276119808Clontech SMART CDS Primer II A (100% over 21bp)
GTGCGGTGGTGTGCACCTGTAATCCCAGCTACTTGGGAGGCTGAGGCAGG26980.22441461306778437No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC24940.2074462731619919No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC24380.20278829750157829No Hit
GGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCCCGCC24310.2022060505440266No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG24170.20104155662892317No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA23950.19921163761947497No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA22250.18507135436464794No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA21360.17766850019006203No Hit
CCTTGTGGTAGCCTTAGGTCATCTGAAAATCCAATTTAAGCAGTTAAAAA21050.17508997794947592No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG20880.17367594962399321No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG20090.16710487681733832No Hit
CGCTAACCGCGAGGCATTAAGATGGATTATACGCTCACCCGCATCTGTCT19150.1592861319587869No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA18990.1579552817700973No Hit
GGTTGGAGTGCAGTGGCATGATCTCTGCCCATTGCAACCTTTGTCTCCTG18800.1563748971710284No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA18670.1552935813927181No Hit
AGGCAGTGAGAAGGACAGGGCCTCACTCCCATGTCAGACTGCTCCTCGGG18380.15288141542571818No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG17310.14398135478885646No Hit
CCCGTACCCAGGTCCTAAGAAAGAGAGCTGGGTCGTCGTCAAGGAGACAC17300.14389817665206336No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17110.14231779205299444No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA16640.13840841962371875No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG16350.13599625365671883No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC16330.13582989738313264No Hit
GAGTGGAGTGGAGCAAACAAACAAAGCGGGCTTTGAAAACTCCGCCTCCC16220.13491493787840853No Hit
ACACCGTCGAAAGTGTCATGCGGAAAATTCTCGGACTGCTGGAACACAGA15460.12859339948213291No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC15230.12668030233589161No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG15160.12609805537833993No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC15080.1254326302839951No Hit
AGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGG14930.1241849582320986No Hit
GCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCT14890.12385224568492621No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA14460.1202755858028229No Hit
GTGAAATTTCCCAGAAGGAGTGCAGAGGGAGGGGAAAAGCAGCCCAGGAC14090.11719799474147818No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG13570.11287273162823698No Hit
GTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGAT13540.11262319721785768No Hit
GAATTGAATTACCTGGTTAGCTGATGGTATTATCAGAAGTCCCTCTTTTA13260.11029420938765087No Hit
GTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCC13070.10871382478858198No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG12810.10655119323196138No Hit
CTCAAGAGGCTGAGGCAGGAGAATTGCTTGAACCTGGGAGGCAGAGGTTG12670.10538669931685796No Hit
ATGTTGCCCAGGTTGGTCTCGAACTCCTGGGCTCAAATGATCCTCCCACC12540.10430538353854767No Hit
CCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTG12220.10164368316116845No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)