FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004047530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1358829
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97060.7142914965753602No Hit
CCTTTAGTCCCAGCCACTTGAGAGGCTGAGGCAGGAGAATTGCCTGAACC85180.6268632771305293No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC84060.6186208860717574No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83990.6181057366305841No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG76820.5653397152989817No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA72610.5343571560512764No Hit
TGCTTGAACCTGGGAGGCAGAGATTACACTGACCTGAGATTGCACCACTG64320.4733487436609021No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC61250.4507557610265898No Hit
TGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGA59940.4411151071989191No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT59430.43736187555608547No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT52440.38592052421607137No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT51030.37554394261529594No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG50540.37193789652708326No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC48440.3564834132918859No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA47090.3465483883549733No Hit
TTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATG45340.33366965232564216No Hit
TTCTTGGACCGTCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATG41850.3079857730442903No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC37670.27722399212851656No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTG37100.2730292038218201No Hit
GAGTACATGGGCAGACGTGTGCTCTTCCGATCTAATCAGAGGCCCTTCTA35740.26302058610759704Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTT34970.2573539422546913No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA34090.25087777785137055No Hit
GGAAGTGAGTCTCCAGGAAGTCACAGAGATGGGGGTCCGTGCGGGCAGAA32150.23660077905314061No Hit
CAAAGAAATGATGCTTCCAACTGCGGCTCCCAGGCATGGCTTTTCTTTCT28780.21180001309951435No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28410.2090770803390272Clontech SMART CDS Primer II A (100% over 21bp)
CTTTGGGAGGCCAAGGCAGGCAGATCACCTGAGGTCAGGAGTTCAAGACC27760.20429354981384706No Hit
GTTGGCAGCCCAGGAAGCCCTGCTGGATGAGAGGCGGCAGGGGCTGGACT27130.19965720484328786No Hit
GGGCCGGAGAGGGGCTGACCGGGTTGGTTTTGATCTGATAAATGCACGCA26980.1985533131836309No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG26660.19619834430969607No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC26560.19546241653659144No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG25800.1898693654609962No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA24190.17802092831401156No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC24160.17780014998208016No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG23580.17353176889807326No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC23040.1695577589233082No Hit
ATCCATCTTAATGCCTCGCGGTTAGCGTTGACGATTAGATGGATTATACG21820.16057944009143169No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA19880.14630244129320172No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19660.14468340019237153No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA19000.13982627688988092No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG18230.13415963303697523No Hit
CGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTTCACGCTCAGGAGA18060.13290855582269734No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC17640.12981765917565788No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG17560.12922891695717414No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC17540.12908173140255322No Hit
GTATTGGTCCATGATCTTCTCCACGAGCTTCTGCTTCTCGCGGCGCAGGG16690.12282634533116382No Hit
CAGTGGTGCAATCTCAGCTCACTGTAACCTCTGCCTCCTGGGCTCAAGCC16670.12267915977654288No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG16350.12032419090260806No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA16320.12010341257067668No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA15560.11451036149508143No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC15480.11392161927659772No Hit
GAGTACATGGGAAGGTTGATGACAACAAGAAACTAGGAGAATGGGTAGGC15240.1121553926211466No Hit
GGCCAGAACATCTTCGCCTGGTGTGGTGGAAAGTCCAACATCCTGGAACG15120.11127227929342103No Hit
ACGCAGAGTACATGGGGCGGGCCGCCGGTGAAATACCACTACTCTGATCG15010.11046275874300591No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG14710.10825497542369203No Hit
GAGCAAGAGTGAATATATTAAATCAGAGACAACAGACTTTAAATCAAAAA14560.10715108376403507No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA13890.102220367684234No Hit
TGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTTGCAA13780.10141084713381891No Hit
GCCGCAGAGGGGTCACACATGGGATGTCCCAGGAGTCCTGGGTGGCTGGG13690.10074851213802473No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)