FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004047531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1659826
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA344492.075458511916309No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC176881.065653869742973No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124710.7513438155565705No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA116320.7007963485329185No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT108190.6518153107614895No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107760.6492246777674286No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC104680.6306685158564813No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT96010.5784341250227434No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA91680.5523470532453402No Hit
AGTCAGGCTGGTCTCGAACTCCTGACCTCAGGTGATCTTGCCTCGGCCTC85120.5128248382661797No Hit
GCACAATTCATCACAATCTGATTAGTCGCCTGCCTCACCTGGGCGGCGGC83660.5040287355421592No Hit
ACAGAAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGC77030.4640847896104772No Hit
TGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGA72260.43534683756008163No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC62360.37570203141775105No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA60420.36401405930501146No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC60310.3633513392367634No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG54460.32810668106174984No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG49070.29563339771759206No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT46750.28165602900544995No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC46310.27900514873245746No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC45780.2758120429490802No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG44200.26629297287787995No Hit
CTTTTGGAGGCCATGCAGGTGGGTCACTTGAGGCCAGGAGTTCAAGATCA43810.26394332899954576No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA43250.2605694813793735No Hit
GTGGTGCACACCTACAATCCCAGCTACTCAGGGGGCTAAGGCAGGAGAAT42000.25303857151291764No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA41530.2502069494031302No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38910.23442216232303867Clontech SMART CDS Primer II A (100% over 21bp)
ATCCATCTTAATGCCTCGCGGTTAGCGTTGACGATTAGGATGGATTATAC38060.22930114361384865No Hit
CCATAGCACATACCCTCTTGAACAGGCTCCTTTTGCTCCCATGTACTCTG36030.21707094599072432No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG35460.21363685109162042No Hit
ATAATCAGCAACTTCATTCTTCTTCCACAAGATTCTTTCATCTTTCTCCT34540.20809410142990892No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG32980.19869552591657197No Hit
ATCAACGCAGAGTACATGGGTTTTTTTTTTAATTATTTCTTTTTCTCATA31540.1900199177504148No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC31070.1871882956406274No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTCTGTCTC31040.18700755380383244No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA30210.18200702965250573No Hit
GAGGGGTGTGTGATGCCATCTCACAGGCAGGGGAAATGTCTTTACCAGCT30120.18146480414212093No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG30090.18128406230532598No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAA28890.17405438883352833No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG28770.17333142148634856No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA28430.17128301400267257No Hit
GCTTTGAACACTCTAATTTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGG27340.16471606059912305No Hit
AGCCTGAGCAACATCATGAAACCCTGTCTCTACAAAAAAAATTTTTTTAA25050.15091943372377586No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24770.14923250991368975No Hit
GGGAGCTGCCTGCGGGAAGGTGCGGGGAGCGGAGCCATGGCCTCCGGTGC24550.1479070697771935No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC23890.1439307493677048No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG23390.14091838542112245No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA22760.1371228068484287No Hit
GGAGTGGTGATGCACGCCTGTAATCCCAGCTACTCAGGAGGCTGAGGCAG22320.13447192657543622No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG20760.12507335106209927No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC20480.12338642725201315No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC20260.12206098711551694No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG19950.12019332146863586No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA19800.11928961228466117No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC19770.11910887044786621No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG19660.11844615037961811No Hit
GCCAGAGCTGATCGCGCAGCCTGGTTGGAGTGGAAATCAGGTGAACGGTG18530.111638207860342No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG17770.1070594146615368No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG17700.10663768370901527No Hit
GTCCAGCCTCAGAACTTCTGGAACTGCTTCTTGGTGCCGGCAGCCTTGGT16950.10211913778914175No Hit
CGCTTGAACCCAGGAGGCAGAGGTTGCAGTAAGCCAAGATCACACCCCTG16810.10127567588409869No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT16620.1001309775843974No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)