FastQCFastQC Report
Fri 9 Sep 2022
EGAF00004047532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2745814
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC302891.1030972964665486No Hit
GTGAAGTACTGTGGTGAATCCTGTGTTTGCTTTTATAACTACTTCTTCCA191280.6966240247882777No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC182590.6649758505128169No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155550.5664986776234661No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129970.47333868936497525No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA126300.4599728896421971No Hit
GAGTCTCTCTGTCCCCCAGGCTGGAGTGTAGTGGCACGATCTTGGCTCAC119540.4353535964198595No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT117650.42847039165799283No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC112740.4105886269062653No Hit
TTGGACAACAGACCATGCCCGGGCACAACAAACCGAGCCGCGACGTGGCG111820.4072380722073673No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT104400.38021512017929837No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA97800.3561785321219864No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT89710.3267155022153722No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA77940.2838502535131659No Hit
CCTGTAATCCCAGCTACTCAGGAGGCTGAGGCAGTAGAATCGCTTGAGCC75570.27521893325622204No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA72720.26483949750420094No Hit
GCCCAGGAGTTTGAGACAGCCTGGGCAATATAGTGAAACCACATCTCTAC72210.26298212479068134No Hit
ACAAAGCGGAAGGCAATATCCAAGCGGCATATGGCAAGCTCAAGGCGATC70860.25806554996077663No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC70110.25533411949971846No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC68300.24874226732036472No Hit
GTGCAATCTTGGCTCACTGCAGCCTCTGCTTCCCAGGTTCCAGTGATTCT65150.23727025938392038No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG64910.23639620163638178No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG63580.23155246495210527No Hit
CCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGC56530.2058770186181584No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC52090.189706950288694No Hit
GATGTGGCTCTGGAAGGCGTGAGCCACTTCTTCCGCGAACTGGCCGAGGA49500.18027441042983974No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG46730.17018632726033153No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC44100.16060811111022086No Hit
GTACATGGGGCCCTGCCTTGTTTCACCTCGGTCTCGATAGCTGTCAATTG43770.15940628170735527No Hit
CTTCAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA42780.15580079349875847No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG42530.15489031667840575No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA42400.1544168687318223No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42230.15379774449398248Clontech SMART CDS Primer II A (100% over 21bp)
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC41180.149973741848501No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC40670.14811636913498147No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG38990.14199796490221114No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC36900.13438637868406236No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG35740.1301617662376257No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG34630.1261192491552596No Hit
CAACGCAGAGTACATGGGAGATTGATAGCTCTTTCTCGATTCCGTGGGTG33060.12040145472344448No Hit
ACCGCACCCAGCCAGGCATCAGTAATTTTTAACATCTCCAAGGTGTTTCT32390.11796137684489919No Hit
AGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTT31170.11351824996157789No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31090.113226897379065No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG29800.10852883698604493No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA29250.10652578798126895No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)