FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004047533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2345626
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA827663.5285250078230717No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC243971.0401061379776655No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA174960.7458989625797121No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA168890.7200210093169158No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160280.6833143902736413No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT156180.6658350478720819No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC153900.6561148281951171No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT134720.5743456117897738No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132590.5652648802494515No Hit
ACCCCCGAGCGGTCGGCGTCCCCTAACTTCTTAGAGGGACAAGTGGCGTT128410.5474444775083496No Hit
TAACAATTGTGAGTGGCTGTTTGAGTTGCCCCCACCATGTCCTTAGATCT106910.4557845112562702No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG102340.436301439359898No Hit
GAATGCAATGGAGTGTGCATTACATTTGGAAAAAAATGTGAATCAGTCAC94830.40428439998533444No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC93730.3995948203166234No Hit
AACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATC91940.39196359521935725No Hit
GAGTACATGGGAGTGACGCGCATGAATGGATGAACGAGATTCCCACTGTC89880.3831812914761347No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC89090.3798133206231514No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC72330.30836117948897224No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG71970.30682640796103045No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT69010.2942071753979535No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG64600.27540622418066646No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA61160.2607406295803338No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA57980.2471834810835146No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC54650.232986844450053No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG51790.22079393731140431No Hit
TTTTTTTTCGGATGGGCTGGATGTGTTTAGTGTAGGTGACAGGTGCAGAC51590.21994128646254776No Hit
GCGAGGACCACCTCGTAAGGCTAAATACTCCTAGGTGACCGATAGGGAAG49510.21107371763443958No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC48760.2078762769512275No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA48150.20527569186221503No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG46860.19977609388709028No Hit
CCAACATGGTGAAACCCCATCTATTAAAAATACAAAAAAAAATTAGCCGG44940.19159064573806736No Hit
TCTTTATCACCAGAATGAGGAACTCCTGGAAGTTAACTGCACCATCAGTG44610.19018377183745405No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC44060.1878389820030985No Hit
CCAACATGGTGAAACCCCATCTATTAAAAATACAAAAAAAATTAGCCGGG43400.1850252342018719No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43110.18378889047102992Clontech SMART CDS Primer II A (100% over 21bp)
GGATGGCTGTTTTGATATTTCTTGAGTTAACCAGAGGAGGAAGCGCACGG42740.18221148640064527No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG40550.17287495960566604No Hit
GTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGC37930.1617052334856452No Hit
CGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCGCGG36050.1536903155063936No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG35910.153093459912194No Hit
GCGTAGGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCC35900.15305082736975117No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC33320.14205163141950164No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA33120.1411989805706451No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC32920.14034632972178854No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32920.14034632972178854No Hit
CCACGACGGTGACTACAAGGACCACGACATCGACTACAAGGACGACGACT31180.13292826733673654No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG29270.12478545173015647No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC28870.12308015003244337No Hit
GAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCT28770.12265382460801509No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA28160.12005323951900261No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG27090.11549155747762005No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG26670.11370099069502129No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC26320.11220885170952233No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA25910.1104609174693664No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT25490.10867035068676764No Hit
CTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGA24880.10606976559775513No Hit
TCCCCCGAGCGGTCGGCGTCCCCTAACTTCTTAGAGGGACAAGTGGCGTT24840.10589923542798382No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)