FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004047534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1208333
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT98350.8139312590155197No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC85400.7067588156576043No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81520.6746484619719895No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA71500.5917243011653245No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69470.5749242965308404No Hit
CTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGA63460.5251863517755453No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT61730.51086910644665No Hit
CCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGG59400.4915863425065773No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC57840.4786759941175156No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT54480.45086908989492136No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC53380.4417656391077625No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG43950.3637242382687554No Hit
GGCTGGAGTGCAGTGGCGCAATCTCGGCTCACTGCAACCTCCACCTCCCG40480.33500698896744524No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC36830.3048000840827818No Hit
GTGATGACTCGAGCTTTCTCCTTCTTATAGACGTTCCGGACGGGCATGAC34940.2891587004575725No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC31280.25886903692938945No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA30860.25539317390156524No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT28580.23652420317909054No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACAC27930.23114489135031482No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG27600.2284138561141672No Hit
GAGCAAATCTCTCTGTTAGTATTAACCAATTCCGCACTTGGTAACCTTGT26490.21922764668348876No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA25990.21508971450750744No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25720.21285523113247756Clontech SMART CDS Primer II A (100% over 21bp)
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC25200.20855178166945704No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA24910.20615178100738787No Hit
CTGGAAGGGCTCATCGCCGGTTTCATTGAGAAGACACGTGCGGATGAGGG23200.19200005296553185No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA23050.19075867331273746No Hit
CCCCCATCTCTACTAAAAATACAAAAATTAGCCGGGCATGGTGACGCGCA22960.19001384552106085No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC22750.1882759140071487No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG22050.1824828089607749No Hit
ACCTAACAAACGGTTTAAAATCTGGCAAGACAAACAGCCCCAGTTGCAGC21930.18148970523853938No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA20870.17271728902545905No Hit
TAGTATCTCTGCCCCTGCCTCCCTAATTAGCTAGGGCTGGGGTCAGCCAC20410.16891039142355627No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG19560.16187590672438806No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC19220.1590621128447208No Hit
GATCCACCATGGGCAAACCGATTCCGTACCCGCTGCTGGGCCTGGATAGC18960.1569103881132105No Hit
GCTTCCAACCAGGCTGCTATGCACCAAAAGCTTGGTCAACCTTTTCTCTG18480.15293797322426847No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA18220.1507862484927582No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG16290.13481383029347044No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG16230.1343172784323527No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15690.12984831168229288No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA15630.12935175982117514No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA15250.12620693136742933No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG15120.12513106900167423No Hit
GTGCTGGGATCACAGGCGTGAGCCACTACGCCCAGCCTATTTTATGTTTT14540.1203310676775359No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC14180.11735175651082937No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC13380.11073106502925932No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG13050.10800002979311166No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC12940.10708968471439578No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG12330.1020414074596986No Hit
ATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCG12310.10187589017265936No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG12200.10096554509394348No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)