FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004047535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2098167
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG503942.401810723359961No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC176950.8433551762085668No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC148160.706140169014192No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129760.6184445756700968No Hit
TGCCTGGCCCATGCCTTTGCTCTTAGTCTTTTCCCTCAGGTCTAAGAGGG126380.602335276457975No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA126260.6017633486752961No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT107120.5105408673380145No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105850.5044879649713297No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA105390.5022955751377274No Hit
CCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCG104220.49671927925660825No Hit
GCTACAATAAATGTCAAACCTAAGCACTTTGCAGTTCACTACTTTTGGGA96380.4593533307882547No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT93300.4446738510328301No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC89530.4267057865270019No Hit
CATCCGCGACGCGGCCGAAGCCGCCGCGGCCTGGGAGGAGATCGGCGAGC86030.4100245595322012No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG76900.3665103874000497No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG73270.34920957197401353No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA66860.31865909624924993No Hit
ATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGTTCTTA65980.3144649591762715No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC65300.3112240350744245No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA64850.30907930588937865No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG58990.2811501658352267No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT50030.23844622472853688No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC49880.23773131500018826No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA47360.22572083156393177No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC45020.21456823980169357No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT37010.17639206030787827No Hit
ACTACGCCCTGCTAATTTTTTTTTTTTGTATTTTTAGTAGAGACGGGGTT36340.17319879685458783No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG35870.1709587463724289No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC35310.16828975005326077No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC35110.1673365370821293No Hit
TCACCATGCCTGGCTAATCTTTTTGTGTTTTTGGTAGAGATGGGGGTTTT34900.16633566346244127No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34860.16614502086821498Clontech SMART CDS Primer II A (100% over 21bp)
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG34350.16371432779182973No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG34120.16261813287502855No Hit
CGAACTAGGTGTTGAAGATCGCGCAGAAACTTTTCGTCACCGGACAGGTG33000.15728014023669232No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG32690.15580266013143854No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC31170.14855824155083938No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT30630.14598456652878442No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC30160.14374451604662544No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG29620.1411708410245705No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA29580.1409801984303442No Hit
GGGCGTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGC29210.13921675443375098No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG28010.13349747660696215No Hit
GTTCCTGTAGCTATCGGGAGGCTGAGATGGGAGGATTGCTTGGGCCCGGG27250.1298752673166626No Hit
GTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCT26040.1241083288413172No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25930.12358406170719491No Hit
GTACATGGGAAGAATTCTTCTGTAATTGTTAGAAGAATTCCTATTGGAGG25250.1203431376053479No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA24270.11567239404680371No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG24090.11481450237278538No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA22480.10714113795517706No Hit
CGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGA22390.1067121921181679No Hit
CACGTGTGGCGTCCGGTACAGTCAGCAGAGTGCAGGGTGCGGGCACCAGG22290.10623558563260217No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT22290.10623558563260217No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC21780.10380489255621692No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG21750.10366191061054719No Hit
CTGTTACCCAGGCTGGTCTCGAATTCCTGGGCTCAAGTGATCCTTCTATC21490.10242273374807628No Hit
CTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAAC21080.1004686471572568No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)