FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004047537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2273968
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC393241.729311934028975No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC194240.8541896807694743No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA168160.7395002920005911No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156970.6902911562519789No Hit
ATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGC144170.6340018856905638No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132670.5834294941705424No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT130410.5734909198370426No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC119810.5268763676533706No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT113800.5004467960850812No Hit
GATTACAGGCGTGAGCCACCGCGCCTGGCCTAAAAAAGGATATTCTTGAA106020.46623347382197106No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA100080.4401117342020644No Hit
CGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGC94930.4174640979996201No Hit
CTGTAGTCCTAGCTATTCCAGAGGCTGAGGTGGGAGGATCACTTAAGACC90470.39785080528837696No Hit
CCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGT88770.390374886541939No Hit
GCTAAATACTTTGCTTGTGAACACACAACTTACCAGTGTGGGGCGATGTT86230.37920498441490824No Hit
GAATGTGGCCGGGCGCAGTGGCTCACGCCTGTAATCCCAGCACTTTGGGA84410.371201353756957No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC83540.3673754423984858No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC79300.3487296215250171No Hit
GTCCTGCTTGGCTGTCGTTATCGTTTTCTGGTGATGTTGTGCTAACAATA70470.30989882003616587No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA64990.28579997607706004No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG63210.27797224938961324No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC58670.2580071487373613No Hit
TCGCCATGTTGGCCAGGCTGGTCTCGAACTCCTGACCTCAAGCGATCTGC58240.25611618105443873No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT57290.2519384617549587No Hit
ATCTCGGCTCACTGTAACATCTGCTTCCCGGGTTCAAACGATTCTCCTGC57080.25101496590981054No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT53640.23588722444643023No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA53250.23417216073401212No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG52030.22880708963362723No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG47120.2072148772542094No Hit
GCAGTGGAGGGGGCAGGACCTCCTGCTGGTGCAGCACCAGCTGCTGGAGC46830.20593957346805233No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG46050.2025094460432161No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA44870.19732027891333562No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA44220.19446183939263878No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC43100.18953652821851497No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG40900.1798618098407717No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG40450.17788289017259698No Hit
ATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAATCCT40450.17788289017259698No Hit
CCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGG40170.176651562379066No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39020.1715943232270639Clontech SMART CDS Primer II A (100% over 21bp)
CTGCAAAGCCGGCAGGAAGAACTGGGGAGCTGAAAGAAAGCCATAAAGAG38280.16834009977273207No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC36670.1612599649599291No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG35130.15448766209550882No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC34660.15242079044208184No Hit
GCGCCAACCCAGCCCCGGGGGGGAGAAGGCGCGACACCACACAGGCAGCG34640.15233283845682966No Hit
ACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCT34480.15162922257481196No Hit
GAGTACATGGGATCAGCACAGAGAACTCTTTCTTTTTGATAGCTGCATAG34350.1510575346706726No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA33950.14929849496562836No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG33930.14921054298037614No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32920.1447689677251395No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC32810.14428523180625233No Hit
GTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGC32530.1430539040127214No Hit
GCCCCAGTCCAGGTCCAGGAGGCTCCCTCCCTGCCCAGCGAGTCTTCCAG32050.14094305636666832No Hit
TCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGT29350.1290695383576198No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA29140.12814604251247158No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCAC28530.12546350696227915No Hit
TCATGGTTCACTGCAGCCTTGACCTCCTGGGCTCAAGCAATCATCCTCAG27820.12234121148582565No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG27200.11961469994300712No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA26330.11578878858453594No Hit
CAGTCATTAAACAGGATTTGGTCACGGCCCACTGGATAGTGGGGAAGCCT26030.11446950880575277No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACC25920.1139857728868656No Hit
CTCCAGCCCCGGGGGTGGCGGGGAGGAGAGAGAGAGAGGGAGAGAGGGAG25800.11345806097535233No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA24820.109148413697994No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC24450.10752130197082808No Hit
GTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCT23640.10395924656811353No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA23620.10387129458286132No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCCATTCGTG23400.10290382274508701No Hit
GTCGCACACCACGCAGATGGGCATCTGGTCCTCCTCGCAGTACAGCTTCA23380.1028158707598348No Hit
GGTAAATAGGCTAGAAAAGCCAAGGCCAAAGGCTGGAGGGGAGAGGACAG22910.10074899910640783No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG22800.10026526318752066No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG22790.10022128719489456No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)