FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004047540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2222445
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT1219915.489044723266493No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA277891.2503796494401436No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC212330.9553892222304714No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA159630.718262994134838No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT143140.6440654324403978No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC141770.6379010504196955No Hit
GTACATGGGAGGCTAAACCGGGTGCGTGTTATAGCCGTCAGGTGTTGCGC132930.598125037964944No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG131870.59335551610951No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT123320.5548843728416226No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114290.5142534460920293No Hit
CGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACA112310.5053443392299922No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101360.45607427855357496No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA88340.39749015161230083No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC76610.3447104427781115No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG74130.33355156145596404No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC72520.32630728769440864No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC72060.3242374951911071No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA67950.30574434912899984No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG65850.29629529639653623No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG65170.2932356031307861No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC57540.2589040448695018No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT57270.25768916666104225No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA55840.2512548117051266No Hit
GGGTCTCACTCTGTCACCCAGACTGGAGTGCAGTGGTGCGATCTCGGCTC54460.24504543419522196No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC50380.22668727460072127No Hit
CAAGTACTCCCTGATAAAGGGGAATTTCCATGCCGTCTACAGGGATGACC46310.20837411049542284No Hit
CCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTT45690.20558439016488597No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG44850.20180476907190054No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC41020.18457149670745507No Hit
GTGTATGTGAGAATGAACGGAAGTGAGATCCAGTATAAAATACTCACGCA37670.16949800782471558No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC36740.16531342732891027No Hit
CCAGAGAGGCTCCCTACTGGGCCCTTCGTGCCATCAGCATCTCCCGGATG34570.15554940617203125No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC32640.14686527675600522No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32520.14632533088557872Clontech SMART CDS Primer II A (100% over 21bp)
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAAT30360.1366063052179019No Hit
ACCTAGGGCAGCTTTCTCTTCTGGCCCCTTGCTCCCAGGACAATCCTTCT29810.1341315533117805No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG29430.1324217247220966No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA27860.12535743291735005No Hit
GCTTAGGACACCCCAAGTGGTTTGGGAAAGGAGGAGGGGAGTGGTGGGTT27430.12342262688165512No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC27220.12247772160840875No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA26910.12108286144314033No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC26560.11950801932106307No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG25980.11689828094733504No Hit
TAGTTCACTGCATCCTGGACCTCCCGGGCTCAAGCGGTCCTCCCACCTCA25580.11509846137924673No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA25570.11505346589004452No Hit
CACCTGAGGTCAGGAGTTCAAGACCAGCCTGGCCAACATGGTGAAACCCC24560.11050892148062157No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24470.1101039620778017No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG24040.10816915604210676No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG24020.10807916506370237No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA23600.10618935451720965No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA23250.10461451239513239No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT22780.10249972440262863No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)