FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004047543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2159489
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA793623.6750360849256465No Hit
GCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCT247331.14531724866392No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC211920.9813432714869119No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA193680.8968788449489671No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA147870.6847453263248853No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137400.6362616341180715No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135440.6271854128453537No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC130610.6048190104232992No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT129860.6013459665689429No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT127520.5905100697433513No Hit
CTTCCACAGCAAACAAAATCCACTTTTAGAACGACAACAACCCAGGGGAA112960.5230867117174479No Hit
AAGTTGATAGCCGCCGAGACATGCAGCACTCCTGCTGCCTGGAAAATGCG103410.47886328663864464No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA96170.4453368366312586No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC94530.43774244740306617No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG85190.3944914746034826No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA82680.3828683545042369No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC77740.3599925723168768No Hit
GTTTGCTTTGGCTCGATGAGAAATCCAGGTCTGAAAGCTTCAGGTACAGC77720.359899957814094No Hit
ACATTGTCATTCTCCTGGCTGGTGGATTCCTGAGTAAGAGCCTGGTCTTC74940.34702654192728No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC73630.3409602919950044No Hit
GTGGAAACAAGGACGAGTGGATAGTCTAGCCCCACATGCAGGAGGTGAGA65690.30419233439021914No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC64500.29868177147464053No Hit
ATCCTGCACCTTGTTGTCATGAACTTTGTTGGCTGAATCATTCTCATTTA61880.28654927161008925No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG61430.28446544529747547No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG57600.2667297680145627No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC56200.2602467528197643No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT55910.25890384252941323No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC52160.24153862325763178No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA48660.22533108527063578No Hit
GAGGTGGGAGGATTGCTCGGACCCTAGAGGCGGAGGTTGCTGTGATTGTG47070.21796823229940043No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG46160.2137542724227815No Hit
GTCTCACTCTGTCACCCAGGCTGGAGTGCAATGGCAGGATCTCAGCCCAC45470.2105590720767737No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45330.20991077055729387Clontech SMART CDS Primer II A (100% over 21bp)
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG44980.2082900167585943No Hit
AGGCAGGAGAATCGCTTGAACCCAGGAGACGGAGGTTGCAGTGAGCCGAG41150.1905543394756815No Hit
ACAAAGCGGAAGGCAATATCCAAGCGGCATATGGCAAGCTCAAGGCGATC40310.18666453035880248No Hit
GTTCGCAGAACATCACCGATCCGCGCCTCGGCCCTGAGCTGCTCGACCAG39460.18272841399053202No Hit
CCGACAGGATTGCCTACTCCCCAGACAGAGCAGCTGCCACACAAGGCAGA38540.17846814686252166No Hit
CGCTTGCTGCGTACGCCAAGATGTGGATGTATTTGTGCTTGTGGTCCTGG38060.17624539879573362No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA37590.174068957980337No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG36270.16795640079666996No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT36080.16707656302023302No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG35750.16554842372431627No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG35340.16364982641726816No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC32570.1508227177818456No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31100.1440155518273073No Hit
CGTCCGGGAATTGCATCTGTTTTTAAGCCTAATGTGGGGACAGCTCATGA28160.13040121991823064No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA27880.12910461687927097No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC26660.12345513220951808No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA25970.1202599318635103No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG24970.11562920672436858No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG24680.11428629643401748No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA24060.11141524684774963No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC23340.10808112474756759No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG22340.10345039960842589No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC21960.10169072405555202No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)