FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004047544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1929877
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC215491.1165996589419949No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC159900.8285502133037494No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA149400.7741426007978747No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144760.7500996177476595No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT134060.6946556697654825No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127630.661337484202361No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC125390.6497305268677745No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT124400.6446006662600777No Hit
ATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCC91800.47567798362279046No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC83600.4331882290943931No Hit
GACAGCCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGAC78890.40878252862747205No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC76830.3981082732215577No Hit
GAGTCTCCCTCTGTTGCCAGGCTGGAGTGCAGTGGTGCGATCTCAGCTCA75320.3902839403754747No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG71580.3709044669686203No Hit
GTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCC70090.3631837676701676No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC63430.3286737963092985No Hit
GTTATATACAGCAGTGACCCGGAGCCCCTCACCCCCACCAGGCTTAGGTG62240.32250760022529934No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT59130.3063925835687974No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC53320.2762870379822134No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG50930.26390282904039997No Hit
GCTCTTTGTTTCCCCTCTTGGATCTGTTGAGTTTCTTTGTTGAAGAAGCC47980.24861688076493996No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG44980.2330718486204043No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA43150.22358937901223755No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC42850.22203487579778403No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG41650.21581686293996977No Hit
CCCTGGATGTGATGAGACCCAGGTGTGGGTCAGGATGTCATTCGTTTGTC40590.21032428491556715No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG39340.20384718818867734No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39230.203277203676711Clontech SMART CDS Primer II A (100% over 21bp)
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA37710.19540105405681293No Hit
CACCAAGAGGGCAGTTTCGTAGTCGAACTCAGGTGGGGAAGACTGTGCGG37340.19348383342565356No Hit
GGAGTGCAGTGGTGTGATCTTGGCTCACTGTAGCCTCTACCTCCCGAGTT37200.1927583985922419No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG35470.18379409672222632No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA35250.18265412769829373No Hit
CCCTAGATGTGATGAGACCCAGGTGTGGGTCAGGATGTCATTCGTTTGTC35110.18192869286488206No Hit
CTTCTGCAGGAGTCAGGATGGAAAGGCAGATGTAAAGTCCCTCATGGCGA34660.1795969380432017No Hit
TTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTGC34340.17793880128111791No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGG33680.17451889420932007No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31810.16482915750589286No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA31060.16094289946975895No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA30590.15850751110044836No Hit
TGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCA29990.15539850467154126No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA28650.14845505698031533No Hit
GTGCTATCCGGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA28080.14550150087285355No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC26740.13855805318162764No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG26560.1376253512529555No Hit
CAGTTGGGCGTTCGAGAATCATGATGCGCCGCGCGCCCTGTCGCGCTGGT24970.1293864842163516No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA24000.1243602571562851No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG23800.12332392167998271No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG23780.12322028813235247No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC23650.12254667007275592No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA23260.1205258158939663No Hit
GAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGC22560.11689864172690799No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG22550.11684682495309287No Hit
GCGTAGGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCC22410.1161213901196812No Hit
GGCAAGAGGACTGCTTGAGCCCAGAAGTTTGAGACCAGCCTGGACAACAT22370.11591412302442072No Hit
GATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTC22060.11430780303615204No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC21770.11280511659551361No Hit
CTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGA21180.10974792694042158No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA21040.10902249210700991No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGT20880.10819342372596803No Hit
TGGTACACATGTATCCCTGGTAGAAGGTGCCCCTGAGGAACATTTTGCAG20480.10612075277336329No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT20030.10378899795168293No Hit
CTATCATGTATGCAGTGACCTACTAAGACTGTAATTGGTACAGTAGATTC19970.10347809730879222No Hit
ACTGTGGCCAACGTGGAGACAGTGGCAGTGTCCCCCACAATCTGCCGCCG19640.10176814377289331No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG19570.10140542635618748No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)