FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004047545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2758411
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA2100207.61380374425711No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA391421.41900536214509No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG253880.92038496076183No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC227790.8258015212381332No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA163830.5939288960202087No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155340.5631503064626701No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT152820.5540146120356974No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA148670.5389697184357226No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG145830.5286739358275472No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC141620.5134115256935968No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133290.4832129802266594No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT126550.45877862291007393No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC89210.32341083326596365No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA84240.3053932137016565No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC84080.3048131696110551No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC83310.30202170742503565No Hit
CTAGGGACCACTTCAGTCTAATTTCATGAGCACAGAACAGTCTTCCCTGG81740.29633002478600906No Hit
GAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGT81310.2947711562925177No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG79120.2868318028024105No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC70380.255146894353307No Hit
GTGTGGTGGCACATACCTGTAATCCCAGCTTTTTGGGAGGCTTAGGCACA69910.2534430148371653No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC66960.24274845191670133No Hit
GTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTT66250.24017450626465744No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA64580.23412029606900495No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC64130.23248892206418842No Hit
GGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACAT60420.21903914971336758No Hit
TTTGGCTACAACACTGGGGTCATCAATGCTCCTGAGAAGATCATAAAGGA59840.21693648988493738No Hit
CGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGG59200.2146163135225316No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT59190.21458006076686903No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG58410.21175234582518707No Hit
TATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGG58360.21157108204687408No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC51180.1855416034811346No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG47700.17292564451055337No Hit
GCCTGGAAAGGCCGTAGCCGAGTTGCCGCTCGTGCCGCATTGAAAGCAGG46050.16694393982622605No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC45870.16629139022429942No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA44360.16081722411924837No Hit
GAAGCTGCCCGGCGGGTCATGGGAATAACGCCGCCGCATCGCCGGTCGGC43850.15896833358045628No Hit
ATCAACGCAGAGTACATGGGAAGCTGTGGGTCTGTGAGTGTTTATGTGTG43830.1588958280691311No Hit
GTTGGTCCTAGCCCATTTCAGCACCCTGCCACTTGGAGTGGACCCCTCCT43650.15824327846720448No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC43460.1575544761096153No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG42970.15577809108214838No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42220.15305913440745414Clontech SMART CDS Primer II A (100% over 21bp)
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA42060.15247909031685272No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG41850.15171778244793832No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG41660.15102898009034912No Hit
TCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGAT39430.14294461557759158No Hit
ACGCAGAGTACATGGGATTCCTGCTTGGAGGCAGACTCTGAGGAGGCTCC38770.14055193370386068No Hit
CAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCC38470.13946435103398297No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG37910.13743419671687793No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC35490.12866102984653122No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACA34730.1259058204161744No Hit
TTAGTAGGTATGGGGTTTCACCATGTTGGCCAGGCTGGTCTTGAACTCTT34030.1233681275197931No Hit
TGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAG31770.11517500474004781No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31020.11245604806535357No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC30900.1120210149974025No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA30890.11198476224173991No Hit
GTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACGAG30560.11078842130487444No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA29970.10864950872078163No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG29190.10582179377909963No Hit
TGTCACATGCCTGCATTCTAGCTGCAAGGGAAGCTAGGAGCAAGTTTAAA29050.10531425519982338No Hit
TACCACCTCTTCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGG28400.10295782608175505No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA27800.10078266074199965No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)