FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004047547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences940167
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCTAGGACATGGAGTGGGGACGGAGGTGGTTCCTCACCTCACGAGATT72910.7755005227794636No Hit
GTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCT57630.6129762052911877No Hit
AAGGGCCACAAAGTGACCAAGAACGTGAGCAAGCCCAGGCACAGCCGACG57590.6125507489626844No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55880.5943624909191665No Hit
TTCCACCACACAGCCACGGCCTCCTGAGAAGACACCACTGACCCCCACCT50660.5388404400494806No Hit
TGCCCACGCGCGTTTCATAGGTGGGGCCGAAGAGGATCGGCAGGCCTTCG50190.5338413281895663No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48980.5209712742523404No Hit
TCTGAGCACTTTGGGAGGCTGAGGCGGGTGGATCTCCAGAGGTCGGGAGT48140.5120366913537702No Hit
GTCCCTCAGCAAGCGTTGTCCTTGTGCTTTGAGACGTATCTAGGAATTGA47490.5051230260155909No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC45790.48704113205419886No Hit
GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTT40960.4356672803874206No Hit
CAGGGTGTATACCACACAAGAATATATGTATAAGTTTGGCCGGGCATGGT40510.430880896691758No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA38720.4118417259912335No Hit
TGGAAAGGCTGGCCTGTTTTGGTACATGGTCTCCCTCCAGGATATGCTCT36230.3853570695419006No Hit
GGCAAGAGAAAGAAACAAAGGTTATTCAATTTAGAAAAGCGGAAGTCAAA33960.36121242289933597No Hit
AAATCACATTGCGTCAGCATCCGCGAGGACCATCGCAATGCTTTGTTTTA33820.3597233257495743No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGTGTGTGCCTACCC32570.3464278154838449No Hit
GCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCG30440.32377226599104203No Hit
TTTATCATTTTCTTCCCTTTAAAAAATACTTGAGGCCAGGCTCAGTGGCT30050.31962406678813443No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT29590.31473131901034607No Hit
ACGCAGAGTACATGGGGGAAGGTTAAGAGGATCCGTTAGCTTCGGTGAAG27050.2877148421503839No Hit
GTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTC26440.28122663314070795No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC26190.2785675310875621No Hit
TTCTCGACCTCGCTGCAACTCGACAAGGCGCTGACCGCTGGAACACCGTC26060.27718479801992624No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA25930.27580206495229037No Hit
GTGCTTCGCTTCCTGTTTCCACCCAACTCCTGACCCCAGTCCAGGCTCTG24710.26282564693293853No Hit
CCTCAGCGTCAGTTACAGACCAGAGAGCCGCTTTCGCCACCGGTGTTCCT24410.25963472446916347No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT22180.23591553415510222No Hit
GTTCTTGGAGGAAACATTGTGAGCGATCTCGGCACAGTAAGATTTGTTGC22130.23538371374447306No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG22110.23517098558022137No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA19610.2085799650487626No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC19450.20687813973474925No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18480.19656082376854322Clontech SMART CDS Primer II A (100% over 21bp)
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT18350.1951780907009074No Hit
GCTGCTGCAGCAGCGGCACTACAAGCCAAATCAGATGAGAAGGCGGCGGT18250.19411444987964904No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG17440.1854989592274564No Hit
GTGCTAGGATTGCAGGTGTGAGCCACTGCGCCCAACCAGAAATGAGACAA17250.18347804166706552No Hit
ATCAACGCAGAGTACATGGGTCTTATCTGGCAATGGGTATATTTAGAGGG17110.18198894451730385No Hit
GTGGAGGCCACAGGAGCAGAAACATGGAATGCCAGACGCTGGGGATGCTG16070.17092707997621698No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC15930.1694379828264553No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG15530.16518341954142188No Hit
GGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAG15500.16486432729504438No Hit
GCTCACTGCAACCTCCACCTCCCAGGTTCAAGTGATTCTCCTGCCTCAGC15480.16465159913079272No Hit
GAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCG15050.16007794359938182No Hit
GGTGAAAGCCATCAAAGAATTGGGTGACCACGTGACCAACTTGCGCAAGA14960.1591206668602493No Hit
GCTTGGGTAGGGACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCC14350.15263245785057333No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC14120.15018608396167915No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA14010.14901607905829498No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT13860.14742061782640745No Hit
ATGAAATGGCACGCGGCTTTCTGGATGAGCTGTTGTCGGGCATCTTCGGA13620.1448678798553874No Hit
GTGCCACACAGCATCCCCCGCCTCGCGCCAAGACTAAAATTAAAAAGCCA13260.14103877289885733No Hit
TAGATATCCTCTTGAATGAGGACAAGTCTCTTGTCCTTTTTCTGTGAATT13130.1396560398312215No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG12680.1348696561355589No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG12680.1348696561355589No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG12470.13263601041091636No Hit
GCTCAGGGGTAGAGCATTTGACTGCAGATCAAGAGGTCCCCAGTTCAAAT12420.1321041900002872No Hit
TTTCTAGCGTGTGAACGTTGAGAAATAATGGGGAATAAAGGATTCCCAAG12380.13167873367178384No Hit
GTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGC12230.1300832724398963No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCCTGTCTCTTATACACAT12140.1291259957007638No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC12120.12891326753651214No Hit
AGGAAGCTCAGCTCGCCCGGCCATTTTAAAGAAGTCAGCAACACAGGGGG12080.12848781120800878No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGT11710.12455234016935288No Hit
GTACATGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTC11600.1233823352659687No Hit
GTTACAGTAAAATCCACTGTGTGTGGAAGGCAGAAGCTAGCGGTTGTATC11600.1233823352659687No Hit
GGATGGAGGAGCAGATCGCCAAGGCCGAGTGGTGGAGTTTGGAGGGGGCC11500.12231869444471034No Hit
CACAAAGTGTGCTTCTCTGGGTGGAGCAGGCTGGCGCTTTAGTTGAACCC11340.12061686913069698No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC11200.11912777198093531No Hit
ATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAA11130.11838322340605445No Hit
TTTCTGAATATGGCATTTTTCCTTTTCTATTTTTTTGAGACAGAGTTTTG11060.11763867483117361No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10880.11572412135290858No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC10800.1148732086959019No Hit
GTACATGGGAGGCGCCCGCCACCACGCCTGGCTAATTTTTTGTACTTTTA10330.10987409683598764No Hit
CACCTGTAGTCCCAGCTACTCAGGTGGCTGAGGCAGGAGAATCGCTTGAA10210.10859772785047762No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGC10040.10678953845433842No Hit
GCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTAC9920.10551316946882841No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC9750.1037049800726892No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG9740.10359861599056337No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG9720.10338588782631171No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA9490.10093951393741749No Hit
AACGCAGAGTACATGGGAGGAGGGCAAGGAAGCACTGGGAGGGACCGGCC9450.10051405760891416No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)