FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004047548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2289169
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT1155655.0483385018755715No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA260291.1370501697340825No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC209160.9136940086118588No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA144250.6301413307623859No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140080.6119251134363606No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG139700.6102651224090488No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT135360.5913062775181736No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC125310.5474038832432206No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT115100.5028025453778205No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114540.500356242811256No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA92630.40464465489441803No Hit
GGTCCCACTCAGTTGGGTCACTTGCTGGACTGCTCCACTGGCCAGGGGCA85920.3753327080700464No Hit
GAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTT79810.34864179970985104No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC77860.3401234247012781No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC73540.3212519477592087No Hit
GTCCCCATGGGCAAACCGTCCGAACCCGCTGCTGGGCCTGGATAGCACCA72510.3167524983957061No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG68430.2989294368393072No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA67890.2965705022215485No Hit
ACAGGGCAGAACCAGGATGGGTGAACCTGAAGTCTCAGGTGTCAAGACAT67860.29643945029833974No Hit
CCTGCAGCTGCTTTTCTAACAAACTGTTGATCAGCAAAAATAAAGGGGCT67470.29473577529662515No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG66410.29010527400991365No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA64780.2829847861822347No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC57640.25179442845853667No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC56060.24489236050287247No Hit
GCTCTGGGGTGTCTGGAGCTAGGCTTGGCCCTACCATTCTGGGGCCATTT55450.24222763806429318No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT52960.23135032843796155No Hit
GAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAA51450.22475404830311785No Hit
CAGTATCCCTGGTGGCTACCCAATAGACGCCAGTAGCACCCCGAATTTAC51140.22339984509662678No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC49190.21488147008805378No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG46300.2022568014856046No Hit
GTATCCCACCTGACCAGCAGCGTCTGATATTTGCCGGCAAACAGCTGGAG45670.1995047110982195No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG43450.18980686878076716No Hit
CCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCGC41720.18224954120905884No Hit
GGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTAT38020.16608647067997165No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC36980.16154333734206605No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36420.1590970347755015Clontech SMART CDS Primer II A (100% over 21bp)
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA35930.15695652002975752No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG34940.15263180656386663No Hit
GTATTGAACCAGCCCTGCATGCCTGAAATAAGTTCCTCATAAAATGTAAT32170.14053134565425268No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG31270.13659978795798824No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC31220.13638136808597356No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC29960.13087718731120332No Hit
GTCGCCCAGGCTGGAGTGCAGTGGCGTGATCTCGGCTCAATGCAAGATCC29010.12672720974292417No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG28910.1262903699988948No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC28900.12624668602449185No Hit
CATCTGTCTCACAGGCCACCTGCCTGCCAGCCCCTCCCAAGTCTTCTGCC28120.12283933602106267No Hit
CTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGG28080.12266460012345091No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27610.12061145332651281No Hit
CCGCAACCTGCTGCTGGTACGGCCCCTGGGCCCCCAGGCCAGCATTCGGC27170.11868935845278353No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACA26610.11624305588621897No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA25950.11335991357562503No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA25390.1109136110090605No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG25350.11073887511144874No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG24360.10641416164555784No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC23580.10300681164212866No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACC23490.1026136558725022No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATG23160.10117208471720524No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)