FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004047549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2185509
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC1641207.509463470523342No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA362151.6570510576712334No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC196320.8982804463399602No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG192420.8804356330722043No Hit
GGTCCTGGCTACTTGGGAGGCTGAGGCAGAAGAATCACTTGAACCCGGGA157350.7199695814567683No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA142210.6506951012327106No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA139530.6384325116025603No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138090.6318436574729274No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC128600.5884212785213879No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT127170.5818781803232108No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117100.5358019573472359No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT109420.5006614019891934No Hit
GTCGCCGCCATGACGACCGCGTCCACCTCGCAGGTGCGCCAGAACTACCA98020.4484996401295991No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG92320.422418759199802No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC84880.38837634619669836No Hit
GCACACAGTGAGAAGTTCTGGGCAGAAGTGAGGGAAAGTTCAGAGGAGCT81860.37455805489705146No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC77370.3540136416734042No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC73290.3353452216394442No Hit
GCTAATTTTTGTATTTTTAGTAGAGACGGGGTTTCACCATATTAGCCAGG71230.3259194997595526No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG67220.307571371245783No Hit
GTGTCACATTGTCATGTAGGCTGGAGTGCAGTGGCATGATCTCGGCTAAC62130.2842816021347887No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC58330.26689434818159063No Hit
TGTCCTGACTGTTCCGTGCCGAACAGTGAGTAGCATAATCGCAGCCGCTG57970.26524713464918237No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGC57430.26277631435057003No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC56850.26012247032613456No Hit
GAACGGGACAGGGCAGTTCTTGCAACCATGGAATCCCTAAATGGTGGCAA56530.2586582805195495No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA51240.2344533927794395No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT51040.23353827415032377No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG46530.212902349063765No Hit
AAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGA43820.20050249163924744No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC39960.18284070209731465No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG39640.1813765122907295No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA39220.17945476316958658No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG37740.17268288531413048No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36930.16897665486621194Clontech SMART CDS Primer II A (100% over 21bp)
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA35700.16334867529715047No Hit
ATATACCAAAATGCAACTTGGTGGTTCGTTTTGATTTGCCCACAGAATAT35550.1626623363253137No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG34040.15575319067549023No Hit
GTACAGGGATAGCACAGCCTGGATAGCAACGTACATGGCTGGGGTGTTGA33980.15547865508675554No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA33160.15172666870738122No Hit
TTGTTGTTTTTTTAATAGTCATTCCAAATATGAGATGCGTTGTTACAGGA31400.14367362477116313No Hit
CGTGGAACCCCAGGTCCGCAGTTATGATAACGGATCACATCGCTCCTGCG31090.14225519089603383No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC30420.13918954348849627No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC30010.1373135502988091No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC29750.1361238960809587No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG27890.1276132928301828No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27270.12477642507992417No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC27130.1241358420395432No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC26720.12225984884985602No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG26450.12102443870054985No Hit
GTGGAGGCCACAGGAGCAGAAACATGGAATGCCAGACGCTGGGGATGCTG26160.1196975166883321No Hit
TGCTTCCGGATAACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA25300.11576250658313465No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG24830.11361197780471277No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA24490.11205627613521611No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA24340.11136993716337933No Hit
GCAATCGCAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGC23520.107617950784005No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC23310.10665707622343353No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC22550.10317962543279391No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACC21950.10043426954544685No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG21890.10015973395671215No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)