FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004047550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1949520
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC1182726.06672411670565No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA267241.3707989658992983No Hit
ATTTGAGACAGAGTCTCGCCCTGTCGCCCAGGCTGGAGTGCAATGGCGTG260931.3384320242931593No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC214311.0992962370224466No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA166610.8546206245639951No Hit
GGCAAGCAGAGCCCATGTAGTTAAGAGGGGATGGGCTGACTTTAGAAAGA160510.8233308711888053No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG144610.741772333702655No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC136820.7018137798022077No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT135450.694786408962206No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120720.6192293487627724No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT120480.6179982764988305No Hit
CAGGGGCGTGAGGTCCCAGGAAGTGGCCAAAAGCTAGACAGATCCCCGTT104950.5383376420862571No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102470.5256165620255242No Hit
ACAATGTCCCAGGCCATGGAAACAGGAATATGGGCTCAAATCCATTTATA89150.45729205137674916No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA84550.43369649965119617No Hit
AGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCC83430.4279514957528007No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC81100.4159998358570315No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC78910.4047663014485617No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG71400.3662439985227133No Hit
ACAGGTACAAGACTGATCTGGAGAAGGACATTATTTCGGACACATCTGGT68750.3526509089416882No Hit
GGCGTAGGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCC64980.33331281546226765No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG61260.3142311953711683No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT60750.3116151668102918No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC58420.2996635069145226No Hit
ACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAAC57160.2932003775288276No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA56180.28817349911773155No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC56140.28796832040707454No Hit
GAGTACATGGGCAGACGTGTGCTCTTCCGATCTCGGATGCAGACATTCTA52230.26791210144035454Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG51460.26396241126020764No Hit
CTATCAATCTGTCAATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCGT48850.2505745003898396No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC48590.24924083877056916No Hit
ACGCAAGGTGATAAATGCATTCTTCTTTAGTAGTTATAGTTTTGTTTTTA46580.23893060856005582No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG44700.22928720915917764No Hit
CTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGA42410.2175407279740654No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC41160.2111288932660347No Hit
GTGTCCCCCAAGTGCTCTCTCGGGGCCAGAAGAGGTTTCCTGGTACCACT38530.19763839304033815No Hit
CTTGGTTCCCCGGAGACGTCCAGTCCGGCGGGCAGCAATGAGACCCACTT34580.17737699536296114No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33530.17199105420821537Clontech SMART CDS Primer II A (100% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG32410.16624605030981984No Hit
CCACTCACCACTAGACACCGGACCTCCGGAGTCCGCACGGACCCCAGGCG32160.1649636833682137No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA30330.15557675735565676No Hit
CGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGG29510.15137059378718865No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG27540.14126554228733226No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA27360.14034223808937585No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT26300.13490500225696583No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA26260.13469982354630883No Hit
TCATAGGGCAGCTGAAAACAAGGGGAAAAAAGACACGTTAAGCTTCCGGA25380.13018589191185523No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC25310.1298268291682055No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24270.12449218269112397No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG24090.12356887849316754No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA23910.12264557429521113No Hit
CTACTAGGGAGGCCGAGGCACAAGAATCACTTGAACCCTGGAGGCGGAGG22790.11690057039681562No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG22450.11515655135623126No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG22200.11387418441462513No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA21990.11279699618367599No Hit
CCTTAGCAGTAGCCTGAGCTGCCCCCAGGGCTGTGAGCTGCTGAATCTTC21770.1116685132750626No Hit
AACGCAGAGTACATGGGGTCCTCTACAGAAGGAAGCACAAAAAGGGACAG21180.10864212729287208No Hit
GGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCA21130.10838565390455085No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC20540.10535926792236038No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC19710.10110180967622799No Hit
GCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAG19520.10012721080060734No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)