FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004047552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2239319
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT1244085.555617578379855No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA288721.2893205478987138No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC234161.0456750467441218No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA169540.757105173492477No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC151800.6778846604704377No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT147850.6602453692394875No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG136860.6111679488272997No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT130700.5836595857937168No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116180.518818444357414No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103160.4606757679455227No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA91450.4083830843216174No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC89470.39954111048939434No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC81270.3629228350226118No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT73170.32675112389079003No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG68040.3038423735073029No Hit
ATGTTGGCCAGGCTGGTCTTGAACTCCTGACCTCAGGTGATCCACCCACC64900.28982025338953493No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC63870.2852206407394391No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG61520.27472637886786117No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG59840.26722409804052033No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC59130.26405349126229893No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA58520.2613294488190383No Hit
GAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATT58370.2606596023165971No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC56010.25012068401152315No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG52230.23324055215000633No Hit
TTGCTGAACTCAGCACATTCGGGACCAATATATAGTGGGTACATCAAGTC45480.20309745954015485No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACC41770.18652992271311056No Hit
GACATGAAGAACTCAGTGGCGGACCGAGACAACAGCCCCAGCTCCTGTGC40580.18121580712707747No Hit
ACATGGGAGATAACCTCGGGCCGATCGCACGCCCCCCCGTGGCGGCGACG40140.1792509240532501No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA38810.17331161839827197No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC38780.17317764909778374No Hit
ACGGCGCCCCCAGTGTGCACGAGTGGGAAGGACTTGGCGCGCCCTCCCTT36650.1636658287631195No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC35470.1583963696105825No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34930.15598492220179438Clontech SMART CDS Primer II A (100% over 21bp)
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA34610.15455591632991994No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC31900.14245402285248326No Hit
GTGGGTCAGAGTGAGAAGATACAGGGAGCTAAGACTGACTAAGGCCTGGG30570.13651471719750513No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG30140.13459449055717387No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG29940.13370136188725232No Hit
GCTTCAATAACATTACGCTTGCCAGCAAGACCTCCCCCAGGAGGCAAATT29920.13361204902026017No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA29680.13254029461635433No Hit
AGCTCACACCTGTGATGTGAGGCACTTTGGGAGGCCAAGGCAAGAGAATT28850.12883381063618002No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC27470.12267122281372149No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG27250.12168878127680781No Hit
ACGCAGAGTACATGGGAGGCGGAGTTTTCAAAGCCCGCTTTGTTTGTTTG27180.12137618624233529No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC26640.11896473883354716No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT26600.11878611309956286No Hit
AGAATTGTGATAGTTCAGCTTGAATGTCTCTTAGAGGGTGGGCTTTTGTT26310.11749107652817666No Hit
GGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCA26040.11628535282378258No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG25430.11356131038052193No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA25300.11298077674507295No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGCCCATGTACTCTGCGTTCTGTCT25180.11244489954312002Illumina Single End PCR Primer 1 (96% over 27bp)
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA24730.1104353600357966No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT24390.10891704129693No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC23880.10663956318863012No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23320.10413880291284985No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG23060.10297773564195187No Hit
GAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTT22490.10043231893267551No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)