FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004047553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2151400
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG1151475.352189272101887No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA223801.0402528586036999No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC191260.8890025099934926No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCAC170440.7922283164451056No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG150010.6972668959747141No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140400.6525983080784605No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA138140.6420935204982802No Hit
CCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTC126370.5873849586315887No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT124590.5791112763781724No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC122520.5694896346565027No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108380.5037649902389142No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT100910.4690434135911499No Hit
GTGGTGCCCTCCCTGGACCAGAGCTGGCATACGGCGGGGAAGGACGGCTG89630.4166124384121967No Hit
GAATGGAATGGAATGGAATGCAATGGAATGGGATCTTCCGGAATGGAATG84930.3947661987542995No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA75040.34879613275076693No Hit
CGTCTATGCAGCCGTCAGAAGCAATGGGGGAAGAACCGTCCAGAGCTGAG74560.34656502742400297No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC73010.33936041647299436No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC71740.3334572836292647No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA69550.3232778655759041No Hit
GAGAGAATGTTCTCTGCATTTTAAAATAGGACAATTAAAAGCAAGATAAA67090.31184345077623876No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG65350.3037556939667193No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA63150.2935297945523845No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT62440.2902296179232128No Hit
ATGCAGTGGCACAATCTTGGCTCACTACAACCTCCGCCTCCCGGGTTCAA62040.28837036348424283No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC61690.28674351585014407No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG60580.2815840847820024No Hit
ACTTTACTATTAATCATCTCAACTCCTGCAGGAAAACTTTTGGCTGCCCC58220.27061448359207957No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA52310.24314399925629823No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT49660.23082643859812213No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC49030.22789811285674444No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC48780.22673607883238822No Hit
GGCCGGGTGGTGGGATGGGCAGGGAGGCGGGGTACCGGCCCCGCTAGAAG47760.22199498001301476No Hit
TCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTC46860.21781165752533235No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG46060.2140931486473924No Hit
GTACATGGGCCGTTGGGATCTATGCGGGTGAGTGTATAATCCATCTAATC44110.20502928325741376No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG42550.1977781909454309No Hit
GATTGTCTCCCATCCAGTCCACGGCCACACCCTCCACATTGTGGATGCCG42500.19754578414055962No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG41950.19498930928697591No Hit
CCTGAAACGAATGCTGGAGAAACTTGGAGTCCCCAAGACTCACCTAGAGC41430.19257227851631495No Hit
TCACCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGT41080.19094543088221624No Hit
TACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGG40860.18992284094078274No Hit
AGCCTGGCCAACATAGTGAAACCCCGTCTTTACTAAAAATACAAAAAAAT36480.16956400483406153No Hit
AGACAGGGCTTCACCATACCGGCCAGGCTGGTCTTGAACTCCTGACCTCG35970.16719345542437483No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC35640.1656595705122246No Hit
ATCAAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC35590.16542716370735336No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG35340.1642651296829971No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34550.16059310216603143Clontech SMART CDS Primer II A (100% over 21bp)
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA33020.15348145393697127No Hit
CCTTTATACTGTGCAGTCTAGGAGAGGTCAGTTTAAAAATAAATATGCAC32900.15292367760528028No Hit
TTTTTGGAGAGTGGAGTGGAGCAAACAAACAAAGCGGGCGTTGAAAACTC32320.15022775866877383No Hit
CGTGGTTTTTCTGCCTCAGCCTCCTGAGTAGCTAAGGGAGGTGTCTTGAG31590.14683461931765363No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG29780.1384214929813145No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG29090.13521427907409128No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA29040.13498187226922004No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC28300.1315422515571256No Hit
GCTCCGAGGTGATTTTCATATTGAATTGCAAATTCGAAGAAGCAGCTTCA27780.12912522078646463No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTA27270.1267546713767779No Hit
GCTCAGGGCAGCTTCCACTGCCAGTGAGAGATGAAGAAAGCTTTCCTGTT26850.12480245421585945No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC26300.12224597936227573No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGC26280.12215301664032722No Hit
CCCCTAACACCAACATAGTCGCTGCACAAAAGCCACAGCCCCTCTCACAC25790.11987542995258903No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25430.11820210095751604No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG24960.1160174769917263No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA24900.11573858882588083No Hit
GTCTTGAACTCCTGGGCTGAAGTGATCTGTCCGTCTCAGCCTCCCAAAAG24050.11178767314306963No Hit
CAAACAGGGCACGTTCGTTTACATAGGAACACCTGCTTAGCATGATAGCA23820.11071860184066189No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG23770.11048619503579064No Hit
AACGCAGAGTACATGGGAACACGGGAAACCTCACCCGGCCCGGACACGGA23350.10853397787487218No Hit
TGGTGACATCACGAATCTTCATGGCATTACGGATGGTCCGGATAAGGTTG23270.10816212698707818No Hit
GTCCCGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC22950.1066747234359022No Hit
TCACCCGTGGTCACCATGGTAGGCACGGCGACTACCATCGAAAGTTGATA22240.1033745468067305No Hit
ACGCAGAGTACATGGGTGTGTGGAGAGTGGAGTGGAGCAAACAAACAAAG22090.10267732639211676No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC22050.10249140094821976No Hit
AAAATTAGCTGGGCGTGGTGGTGTGCGCCTATAATCCCAGCTGCTTGGGA21910.10184066189458027No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)